Zhang Jing, Chen Benshou, Liu Baosi, Zhou Xianrong, Mu Jianfei, Wang Qiang, Zhao Xin, Yang Zhennai
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 102488, China.
Environmental and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, China.
Medicina (Kaunas). 2018 Nov 19;54(5):89. doi: 10.3390/medicina54050089.
Paocai (pickled cabbage), which is fermented by lactic acid bacteria, is a traditional Chinese food. The microorganisms of Paocai were isolated and identified, and the constipation inhibition effect of one of the isolated was investigated. The 16S rDNA technology was used for microbial identification. A mouse constipation model was established using activated carbon. After intragastric administration of (10⁸ CFU/mL), the mice were dissected to prepare pathological sections of the small intestine. Serum indicators were detected using kits, and the expression of small intestine-related mRNAs was detected by qPCR assay. One strain of was identified and named CQPC03 (LF-CQPC03). Body weight and activated carbon propulsion rate were all higher in mice intragastrically administered with LF-CQPC03 compared with the control group, while the time to the first black stool in treated mice was lower than that in the control group. Serum assays showed that gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels were significantly higher in the LF-CQPC03-treated mice than in the control group, while somatostatin (SS) levels were significantly lower than in the control mice. Mouse small intestine tissue showed that c-Kit, stem cell factor (SCF), and glial cell-derived neurotrophic factor (GDNF) mRNA expression levels were significantly higher in the LF-CQPC03 treated mice than in control mice, while transient receptor potential cation channel subfamily V member 1 (TRPV1) and inducible nitric oxide synthase (iNOS) expression levels were significantly lower in the LF-CQPC03 treated mice than in control mice. There is a better effect with high-dose LF-CQPC03, compared to the lower dose (LF-CQPC03-L), showing good probiotic potential, as well as development and application value.
泡菜是一种传统的中国食品，由乳酸菌发酵而成。对泡菜中的微生物进行了分离和鉴定，并研究了其中一种分离菌的便秘抑制作用。采用16S rDNA技术进行微生物鉴定。用活性炭建立小鼠便秘模型。以10⁸CFU/mL的剂量灌胃后，解剖小鼠以制备小肠病理切片。使用试剂盒检测血清指标，并通过qPCR检测小肠相关mRNA的表达。鉴定出一株菌并命名为CQPC03（LF-CQPC03）。与对照组相比，灌胃LF-CQPC03的小鼠体重和活性炭推进率均较高，而处理组小鼠首次排黑便的时间低于对照组。血清检测显示，LF-CQPC03处理组小鼠的胃泌素（Gas）、内皮素（ET）和乙酰胆碱酯酶（AchE）水平显著高于对照组，而生长抑素（SS）水平显著低于对照小鼠。小鼠小肠组织显示，LF-CQPC03处理组小鼠的c-Kit、干细胞因子（SCF）和胶质细胞源性神经营养因子（GDNF）mRNA表达水平显著高于对照组，而瞬时受体电位阳离子通道亚家族V成员1（TRPV1）和诱导型一氧化氮合酶（iNOS）表达水平显著低于对照组。与低剂量（LF-CQPC03-L）相比，高剂量LF-CQPC03效果更好，具有良好的益生菌潜力以及开发应用价值。
