Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 194 Xuefu Road, Harbin, 150081, Heilongjiang, China.
Biol Trace Elem Res. 2021 Aug;199(8):2972-2982. doi: 10.1007/s12011-020-02407-1. Epub 2020 Sep 30.
Acrylamide (AA) is an organic chemical widely existing in the public diet, especially in foods with high-temperature fried and baked starchy and may have various adverse health effects on organisms. The purpose of this study was to investigate whether quercetin plays a protective role in AA-induced element variation in rats. Rats were randomly divided into the control group, AA-treated group [5 mg/kg body weight (bw)], two dosages of quercetin-treated groups (10 and 50 mg/kg·bw, respectively), and two dosages of quercetin plus AA-treated groups. After a 16-week treatment, the serum samples of rats were collected. Serum elements were analyzed by using inductively coupled plasma mass spectrometry (ICP-MS) combined with multivariate statistical analysis, and antioxidant indices, lipid peroxidation indicator, as well as inflammatory biomarkers, were also detected. The accuracy and precision of the method were verified, and all the validated data are within the satisfactory range. The results showed that the levels of vanadium (V), copper (Cu), zinc (Zn), selenium (Se), cobalt (Co), and magnesium (Mg) in serum were significantly lower (p < 0.01), while serum calcium (Ca) level was significantly higher (p < 0.01) in AA-treated group compared with the control group. When high-dose quercetin was administered to rats combined with AA, a significant recovered effect for the above elements levels was observed compared with the AA-treated group. This study suggests that quercetin (50 mg/kg·bw) exerts a regulatory and protective role in AA-induced variation of serum elements via reducing oxidative stress and inhibiting inflammation.
丙烯酰胺（AA）是一种广泛存在于公众饮食中的有机化学物质，尤其存在于高温煎炸和烤制的淀粉类食品中，可能对生物体产生各种不良健康影响。本研究旨在探讨槲皮素是否在 AA 诱导的大鼠元素变化中发挥保护作用。大鼠随机分为对照组、AA 处理组[5mg/kg 体重（bw）]、槲皮素处理组（10 和 50mg/kg·bw，分别）和槲皮素加 AA 处理组。经过 16 周的治疗后，收集大鼠的血清样本。采用电感耦合等离子体质谱（ICP-MS）结合多变量统计分析对血清元素进行分析，并检测抗氧化指标、脂质过氧化指标和炎症生物标志物。验证了方法的准确性和精密度，所有验证数据均在满意范围内。结果表明，与对照组相比，AA 处理组大鼠血清中钒（V）、铜（Cu）、锌（Zn）、硒（Se）、钴（Co）和镁（Mg）的水平显著降低（p<0.01），而血清钙（Ca）水平显著升高（p<0.01）。当高剂量槲皮素与 AA 联合给药时，与 AA 处理组相比，上述元素水平的恢复作用显著。本研究表明，槲皮素（50mg/kg·bw）通过降低氧化应激和抑制炎症，对 AA 诱导的血清元素变化发挥调节和保护作用。
