Ansar Sabah, Siddiqi Nikhat Jamal, Zargar Seema, Ganaie Majid Ahmad, Abudawood Manal
Clinical Laboratory Sciences, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia.
Biochemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
BMC Complement Altern Med. 2016 Aug 30;16(1):327. doi: 10.1186/s12906-016-1322-7.
Quercetin (QR), is a polyphenolic flavonoid compound which is found in large amounts in certain foods, and protects against oxidative stress. The current study was conducted to determine whether Quercetin can possibly exert hepatoprotective and antioxidant activity against acrylamide (ACR) induced toxicity in rats.
Four groups of Wistar rats consisting of six rats each: (i) control group; (ii) ACR treated group (50 mg/kg bw); (iii) QR group: rats were treated with QR (10 mg/kg bw); (iv) QR (10 mg/kg bw) was given i.p. for 5 days followed by ACR (50 mg/kg bw) on 5th day (single dose).
ACR caused an elevation in 8-OH guanosine level and a reduction in Glutahione S-transferase (GST) activity. Administration of QR significantly protected liver tissue against hepatotoxic effect of acrylamide from amelioration of the marker enzyme (p < 0.05) and DNA damage (p < 0.01) as evident by comet assay and, besides some indices of histopathological alterations.
It is concluded that QR could protect the liver against DNA damage induced by ACR probably is thus capable of ameliorating ACR-induced changes in the rat livers.
槲皮素（QR）是一种多酚类黄酮化合物，在某些食物中含量丰富，具有抗氧化应激作用。本研究旨在确定槲皮素是否可能对丙烯酰胺（ACR）诱导的大鼠毒性发挥肝脏保护和抗氧化活性。
将四组Wistar大鼠，每组六只：（i）对照组；（ii）ACR处理组（50mg/kg体重）；（iii）QR组：大鼠用QR（10mg/kg体重）处理；（iv）第1至5天腹腔注射QR（10mg/kg体重），第5天给予ACR（50mg/kg体重，单次剂量）。
ACR导致8-羟基鸟苷水平升高，谷胱甘肽S-转移酶（GST）活性降低。QR给药显著保护肝组织免受丙烯酰胺的肝毒性作用，改善标志物酶（p<0.05）和DNA损伤（p<0.01），彗星试验及一些组织病理学改变指标也证明了这一点。
得出结论，QR可能保护肝脏免受ACR诱导的DNA损伤，因此能够改善大鼠肝脏中ACR诱导的变化。
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