Zhang Shan, Di Na, Tayier Baihetiya, Guan Lina, Wang Guodong, Lu Hanbing, Yan Fei, Mu Yuming
Department of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830011, China.
Biomater Sci. 2020 Oct 7;8(19):5402-5414. doi: 10.1039/d0bm01125h. Epub 2020 Sep 2.
Ovarian survival after transplantation is key to determining the success and efficacy of ovarian tissue cryopreservation and transplantation (OTCP). However, non-invasive monitoring of ovarian survival in the early stages of ovarian transplantation remains a great challenge. Anti-Müllerian hormone (AMH) is a survival factor that can promote the growth of follicles and has been recognized as an ovarian tissue-specific marker. In this study, we developed AMH-targeted nanobubbles (NB) by integrating an AMH antibody onto the surface of NBs. The resulting NB exhibited a high affinity for ovarian granulosa cells in vitro and a significantly enhanced ultrasound signal in transplanted ovaries relative to the non-targeted NBs. Notably, the difference in enhanced ultrasonic signals became more significant with the increase in time after transplantation from 3 to 10 days, indicating a gradually enhanced AMH expression along with the increase in transplant time. These results were further confirmed by immunohistochemical staining and western immunoblotting analyses. In conclusion, our study offers a promising non-invasive tool to monitor ovarian survival in the early stages following transplantation.
卵巢移植后的存活情况是决定卵巢组织冷冻保存与移植（OTCP）成败及疗效的关键。然而，在卵巢移植早期对卵巢存活情况进行非侵入性监测仍是一项巨大挑战。抗苗勒管激素（AMH）是一种能促进卵泡生长的存活因子，已被视为卵巢组织特异性标志物。在本研究中，我们通过将AMH抗体整合到纳米气泡（NB）表面，开发出了靶向AMH的纳米气泡。所得纳米气泡在体外对卵巢颗粒细胞表现出高亲和力，且相对于非靶向纳米气泡，移植卵巢中的超声信号显著增强。值得注意的是，随着移植后时间从3天增加到10天，增强超声信号的差异变得更加显著，表明随着移植时间增加，AMH表达逐渐增强。这些结果通过免疫组织化学染色和western免疫印迹分析得到进一步证实。总之，我们的研究提供了一种有前景的非侵入性工具，用于监测移植后早期的卵巢存活情况。
