Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China.
Poult Sci. 2021 Mar;100(3):100959. doi: 10.1016/j.psj.2020.12.056. Epub 2020 Dec 26.
To elucidate the role of anti-müllerian hormone (AMH) in regulating the development of ovarian follicles in laying hens, the expressions of follicle-stimulating hormone receptor (FSHR), AMH receptor type 2 (AMHR2), steroidogenic-related genes steroidogenic acute regulatory protein (STAR), cytochrome P450 side-chain cleavage (CYP11A1), and 3β-hydroxysteroid dehydrogenase (HSD3B1) genes were measured from different sized follicles and granulosa cells. The results showed that the expressions of FSHR and AMHR2 genes were higher in small follicles and decreased after follicular selection. Oppositely, the expressions of STAR, CYP11A1, and HSD3B1 were significantly increased after follicular selection. It indicated that AMHR2 might mediate AMH suppression in the stimulating effects of follicle-stimulating hormone (FSH) on steroidogenic-related genes expression. To make sure the effects of AMH in this process, a total of 40 hens were treated (negative control, sham operation, 150 ng AMH/d or 300 ng AMH/d) for 25 d. We analyzed ovarian morphology, progesterone concentration in blood plasma, and the expressions of steroidogenic genes in ovaries and follicles. The AMH300 group had significantly lower weight of ovary and hierarchical follicles. Egg weight and ovary weight in AMH150 group were higher than those of sham operation and AMH300 groups, so did hierarchical follicles weight. The steroidogenic genes expressions showed an increase in ovarian tissue and the largest follicle of AMH150 and AMH300 groups. However, progesterone level in the blood was reduced by AMH injection with different concentrations. To further verify the above results, granulosa cells from 6 to 8 mm follicles were cultured with AMH (0, 5, 10, 20, 40, or 80 ng/mL). The results revealed that excessive AMH (80 ng/mL) exerted an inhibitory effect on progesterone synthesis and the expressions of STAR, CYP11A1, and HSD3B1. However, these genes expressions showed a significant increase in 20 ng/mL AMH-treated group. In summary, AMH inhibited the development of prehierarchical follicles in laying hens. The effects of AMH treatment with different concentrations on follicle development showed the follicle was selected by changing FSH responsiveness of prehierarchical follicles.
为了阐明抗苗勒管激素（AMH）在调节产蛋母鸡卵巢卵泡发育中的作用，测量了不同大小卵泡和颗粒细胞中卵泡刺激素受体（FSHR）、AMH 受体 2 型（AMHR2）、类固醇生成相关基因类固醇急性调节蛋白（STAR）、细胞色素 P450 侧链裂解酶（CYP11A1）和 3β-羟甾脱氢酶（HSD3B1）的表达。结果表明，FSHR 和 AMHR2 基因在小卵泡中的表达较高，并且在卵泡选择后降低。相反，STAR、CYP11A1 和 HSD3B1 的表达在卵泡选择后显著增加。这表明 AMHR2 可能介导 AMH 对卵泡刺激素（FSH）对类固醇生成相关基因表达的刺激作用的抑制。为了确定 AMH 在这个过程中的作用，共对 40 只母鸡进行了处理（阴性对照、假手术、150ng AMH/d 或 300ng AMH/d）25d。我们分析了卵巢形态、血浆中孕酮浓度以及卵巢和卵泡中类固醇生成基因的表达。AMH300 组的卵巢和分级卵泡重量明显降低。AMH150 组的蛋重和卵巢重高于假手术和 AMH300 组，分级卵泡重也是如此。在 AMH150 和 AMH300 组中，卵巢组织和最大卵泡中的类固醇生成基因表达均增加。然而，不同浓度 AMH 注射后，血液中的孕酮水平降低。为了进一步验证上述结果，用 AMH（0、5、10、20、40 或 80ng/ml）培养 6-8mm 卵泡的颗粒细胞。结果表明，过量的 AMH（80ng/ml）对孕酮合成和 STAR、CYP11A1 和 HSD3B1 的表达有抑制作用。然而，在 20ng/ml AMH 处理组中，这些基因的表达显著增加。综上所述，AMH 抑制了产蛋母鸡前卵泡的发育。不同浓度 AMH 处理对卵泡发育的影响表明，卵泡是通过改变前卵泡对 FSH 的反应性而被选择的。
