Hao Bingjie, Song Tao, Ye Mao, Liu Xuanyong, Qiu Jiajun, Huang Xiaoyu, Lu Guolin, Qian Wenhao
Department of Stomatology, Shanghai Xuhui District Dental Center, 500 Fenglin Road, Shanghai 200032, People's Republic of China.
Biomater Sci. 2020 Nov 7;8(21):6037-6044. doi: 10.1039/d0bm01286f. Epub 2020 Sep 30.
Hydrogen peroxide (HO) is an important mediator in biological medicine, disease diagnosis and environmental analyses and therefore it is essential to develop a detection approach for HO in physical environments. Herein, we designed and prepared a series of AuNP-containing nanocomposites (AuNPs@NGO-PEG, AuNPs@G1-PAMAM-NGO-PEG and AuNPs@G3-PAMAM-NGO-PEG) for enhanced non-enzymatic HO detection. We firstly demonstrated functionalized nanographene oxide (NGO) based materials, which combined advantages of biocompatible poly(ethylene glycol) (PEG), hyperbranched polyamidamine (PAMAM) dendrimer and thiol active site, as compatible platforms. Gold nanoparticles (AuNPs) were then aptly in situ grown on these functionalized NGO based materials via the reduction of HAuCl under mild conditions, i.e. AuNPs@NGO-PEG, AuNPs@G1-PAMAM-NGO-PEG and AuNPs@G3-PAMAM-NGO-PEG nanocomposites, which possess stable and uniform AuNPs standing on the functionalized NGO sheets. For HO detection, these nanocomposites were cast on a glassy carbon electrode (GCE) conveniently, i.e. GCE/AuNPs@NGO-PEG, GCE/AuNPs@G1-PAMAM-NGO-PEG and GCE/AuNPs@G3-PAMAM-NGO-PEG. It is evident that these GCEs could be applied as efficient non-enzymatic HO detectors resulting from the corresponding cyclic voltammetric curves and typical ready-state amperometric curves. GCE/AuNPs@G1-PAMAM-NGO-PEG exhibited the fastest electron transfer rate among these modified GCEs. We envisage that these GCEs could provide efficient sensors for HO detection and a new strategy for sensor design.
过氧化氢（HO）是生物医药、疾病诊断和环境分析中的一种重要介质，因此开发一种在物理环境中检测HO的方法至关重要。在此，我们设计并制备了一系列含金纳米粒子的纳米复合材料（AuNPs@NGO-PEG、AuNPs@G1-PAMAM-NGO-PEG和AuNPs@G3-PAMAM-NGO-PEG），用于增强非酶法HO检测。我们首先展示了基于功能化氧化石墨烯（NGO）的材料，该材料结合了生物相容性聚乙二醇（PEG）、超支化聚酰胺胺（PAMAM）树枝状大分子和硫醇活性位点的优点，作为兼容平台。然后，通过在温和条件下还原HAuCl，使金纳米粒子（AuNPs）适当地原位生长在这些基于功能化NGO的材料上，即AuNPs@NGO-PEG、AuNPs@G1-PAMAM-NGO-PEG和AuNPs@G3-PAMAM-NGO-PEG纳米复合材料，这些复合材料在功能化的NGO片上具有稳定且均匀的AuNPs。对于HO检测，这些纳米复合材料方便地被浇铸在玻碳电极（GCE）上，即GCE/AuNPs@NGO-PEG、GCE/AuNPs@G1-PAMAM-NGO-PEG和GCE/AuNPs@G3-PAMAM-NGO-PEG。显然，由于相应的循环伏安曲线和典型的稳态安培曲线，这些GCE可以用作高效的非酶法HO探测器。GCE/AuNPs@G1-PAMAM-NGO-PEG在这些修饰的GCE中表现出最快的电子转移速率。我们设想这些GCE可以为HO检测提供高效的传感器，并为传感器设计提供一种新策略。
