Walliker D, Quakyi I A, Wellems T E, McCutchan T F, Szarfman A, London W T, Corcoran L M, Burkot T R, Carter R
Science. 1987 Jun 26;236(4809):1661-6. doi: 10.1126/science.3299700.
Malaria parasites are haploid for most of their life cycle, with zygote formation and meiosis occurring during the mosquito phase of development. The parasites can be analyzed genetically by transmitting mixtures of cloned parasites through mosquitoes to permit cross-fertilization of gametes to occur. A cross was made between two clones of Plasmodium falciparum differing in enzymes, drug sensitivity, antigens, and chromosome patterns. Parasites showing recombination between the parent clone markers were detected at a high frequency. Novel forms of certain chromosomes, detected by pulsed-field gradient gel electrophoresis, were produced readily, showing that extensive rearrangements occur in the parasite genome after cross-fertilization. Since patients are frequently infected with mixtures of genetically distinct parasites, mosquito transmission is likely to provide the principal mechanisms for generating parasites with novel genotypes.
疟原虫在其大部分生命周期中是单倍体，合子形成和减数分裂发生在蚊子发育阶段。通过将克隆寄生虫混合物通过蚊子传播以允许配子发生交叉受精，从而可以对寄生虫进行遗传分析。在恶性疟原虫的两个克隆之间进行了杂交，这两个克隆在酶、药物敏感性、抗原和染色体模式方面存在差异。在高频下检测到了在亲本克隆标记之间显示重组的寄生虫。通过脉冲场梯度凝胶电泳检测到的某些染色体的新形式很容易产生，这表明交叉受精后寄生虫基因组中发生了广泛的重排。由于患者经常感染基因不同的寄生虫混合物，蚊子传播很可能是产生具有新基因型寄生虫的主要机制。
