Electrodeposition of poly-ethylenedioxythiophene-graphene oxide nanocomposite in a stainless steel tube for solid-phase microextraction of letrozole in plasma samples.

Coated stainless steel was used as an in-tube solid-phase microextraction for the extraction of letrozole from plasma samples. The coating process on the inner surface of the stainless steel was conducted by a simple electrodeposition process. The coated composite was prepared from 3,4-ethylenedioxythiophene and graphene oxide. In this composite, graphene oxide acts as an anion dopant and sorbent. The coated nanostructured polymer was characterized using different techniques. The operational factors affecting the extraction process, including pH, adsorption, and desorption time, the recycling flow rate of the sample solution, sample volume, desorption solvent type and its volume, and ionic strength were optimized to achieve the best extraction efficiency of the analyte. The total extraction time including adsorption and desorption steps was about 15.0 min. The developed method demonstrated a linear range of 5.0-1500.0 μg/L with a limit of detection of 1.0 μg/L. The repeatability of the developed extraction approach in terms of intraday, interday, and fiber to fiber was attained in the range of 4.9-8.3%. After finding the optimal conditions, the potential of the described approach for letrozole quantitation was investigated in plasma samples, and satisfactory results were obtained.