Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand.
Pharm Biol. 2020 Dec;58(1):1023-1031. doi: 10.1080/13880209.2020.1822419.
Bambara groundnut (BG), originally from Africa, is widely distributed in Asian countries, especially in southern Thailand, and is used for food and functional foods. There is no report on the use of BG for ethnomedicine or cosmetics.
To investigate collagen biosynthesis stimulation and anti-melanogenesis of the BG extracts.
The hulls (H) and seeds (S) of BG were collected from Trang province, Thailand and extracted by Soxhlet (S) and maceration (M) using ethanol, and boiled with distilled-water (B). Total phenolic (TPC) and total flavonoid (TFC) contents were quantified. The three antioxidant and tyrosinase inhibition activities were determined by DPPH, FIC and FTC; and the modified dopachrome methods, respectively. The collagen biosynthesis and the anti-melanogenesis activities were investigated by Sirius-Red and the melanin content assay.
The yields of BG extracts ranged from 1.72% to 9.06%. The BG-SS extract gave the highest TPC and TFC. The BG-HM extract showed the highest antioxidant activities (SC of 0.87 ± 0.02 mg/mL, MC of 1.83 ± 0.09 mg/mL and LC of 0.70 ± 0.06 mg/mL), tyrosinase inhibition activity (IC of 0.45 ± 0.23 mg/mL), and anti-melanogenesis activities (72.9 ± 0.08%), whereas the BG-SB extract exhibited the highest stimulation of collagen biosynthesis (18.04 ± 0.03%). All BG extracts at 0.1 mg/mL showed no cytotoxicity on human dermal fibroblasts.
The biological activities of BG extracts might be from their phytochemicals, especially phenolic and flavonoid contents.
The BG-HB and BG-HM extracts might be promising novel active sources for anti-aging and whitening cosmeceuticals.
原产于非洲的斑豆（BG）广泛分布于亚洲国家，尤其在泰国南部，被用于食品和功能性食品。目前尚无 BG 用于民族医学或化妆品的报道。
研究 BG 提取物对胶原蛋白生物合成的刺激作用和抗黑色素生成作用。
从泰国 Trang 省收集 BG 的种皮（H）和种子（S），用索氏提取法（S）和浸渍提取法（M）用乙醇提取，以及用水煮提取法（B）提取。用 Folin-Ciocalteu 法测定总酚（TPC）和总黄酮（TFC）含量。通过 DPPH、FIC 和 FTC 法测定三种抗氧化和酪氨酸酶抑制活性；通过改良多巴色素法分别测定胶原蛋白生物合成和抗黑色素生成活性。
BG 提取物的产率范围为 1.72%至 9.06%。BG-SS 提取物的 TPC 和 TFC 最高。BG-HM 提取物具有最高的抗氧化活性（SC 为 0.87±0.02mg/mL，MC 为 1.83±0.09mg/mL，LC 为 0.70±0.06mg/mL）、酪氨酸酶抑制活性（IC 为 0.45±0.23mg/mL）和抗黑色素生成活性（72.9±0.08%），而 BG-SB 提取物对胶原蛋白生物合成的刺激作用最强（18.04±0.03%）。所有 BG 提取物在 0.1mg/mL 时对人真皮成纤维细胞均无细胞毒性。
BG 提取物的生物活性可能来自其植物化学物质，尤其是酚类和类黄酮含量。
BG-HB 和 BG-HM 提取物可能是有前途的新型抗衰老和美白化妆品的活性物质来源。
