Preventive Effect of on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell.

Whitening research is of particular interest in the cosmetics market. The main focus of whitening research is on melanogenesis inhibition through tyrosinase activity. The mechanism of melanogenesis is involved with tyrosinase activity and p-PKC signaling. In this study, we used () , a tropical fruit found throughout Southeast Asia, to investigate the inhibitory effect of melanogenesis. contains a high concentration of polyphenols, flavonoids, and unsaturated fatty acids, which might be related to antioxidant activity. This study aimed to determine whether extracts inhibit melanin synthesis in melan-A cells by inhibiting tyrosinase activity and p-PKC signaling. was divided into pulp and aril and extracted under various conditions, and it was confirmed that all pulp and aril extracts have high contents of both phenols and flavonoids. Melan-A cells were treated with PMA for three days to induce melanin synthesis. After PMA treatment, extracts were added to cultured media in a dose-dependent manner. Melanin contents and MTS were used to determine the amount of melanin in live cells. extracts were evaluated for their effects on tyrosinase activity and p-PKC signaling pathways by Western blotting. It was found that extract treatment decreased the amount of melanin and suppressed p-PKC expression. Additionally, tyrosinase activity was reduced after extract treatment in a dose-dependent manner. Therefore, it was concluded that could be used in antimelanogenesis and functional cosmetic materials to improve whitening.