School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 110, Taiwan.
GeneFerm Biotechnology Co., Ltd., Tainan 741, Taiwan.
Molecules. 2020 Sep 28;25(19):4450. doi: 10.3390/molecules25194450.
This study investigated the physicochemical characteristics of potato protein isolate hydrolysate (PPIH) and its antioxidant activity. Potato protein isolate (PPI) was hydrolyzed into PPIH by the proteases bromelain, Neutrase, and Flavourzyme. Compared with PPI, the resulting PPIH had a lower molecular weight (MW, from 103.5 to 422.7 Da) and smaller particle size (<50 nm), as well as a higher solubility rate (>70%) under acidic conditions (pH 3-6). PPIH presented good solubility (73%) across the tested pH range of 3-6. As the pH was increased, the zeta potential of PPIH decreased from -7.4 to -21.6. Using the 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging assay, we determined that the half-maximal effective concentration (EC) values of ascorbic acid, PPIH, and PPI were 0.01, 0.89, and >2.33 mg/mL, respectively. Furthermore, PPIH (50 μg/mL) protected C2C12 cells from HO oxidation significantly better than PPI (10.5% higher viability rate; < 0.01). These findings demonstrated the possible use of PPIH as an antioxidant in medical applications.
本研究旨在探讨马铃薯蛋白分离物水解产物（PPIH）的理化特性及其抗氧化活性。采用菠萝蛋白酶、中性蛋白酶和风味蛋白酶将马铃薯蛋白分离物（PPI）水解成 PPIH。与 PPI 相比，所得 PPIH 的分子量（MW，从 103.5 至 422.7 Da）更低，粒径更小（<50nm），在酸性条件（pH 3-6）下的溶解度更高（>70%）。PPIH 在测试的 pH 范围 3-6 内具有良好的溶解度（73%）。随着 pH 值的升高，PPIH 的zeta 电位从-7.4 降低至-21.6。通过 2,2'-连氮基-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除试验，我们确定抗坏血酸、PPIH 和 PPI 的半最大有效浓度（EC）值分别为 0.01、0.89 和>2.33 mg/mL。此外，PPIH（50μg/mL）对 C2C12 细胞的 HO 氧化具有更好的保护作用，细胞活力比 PPI（高 10.5%；<0.01）。这些发现表明 PPIH 可能作为一种抗氧化剂在医学应用中使用。
