Mahobia Swati, Bajpai Jaya, Bajpai Anil Kumar
Bose Memorial Research Laboratory, Department of Chemistry, Government Autonomous, Science College Jabalpur, (M.P.) - 482001 India.
Iran J Pharm Res. 2016 Fall;15(4):695-711.
The aim of the present work was to prepare and characterize biopolymer nanocarriers and evaluate their suitability in possible oral delivery of insulin. The egg albumin biopolymer was used to prepare nanoparticles which were further characterized by Fourier transformed Infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), zeta potential, Dynamic Light scattering (DLS) and cytotoxicity. From the characterization studies the size of the nanoparticles washemoly found to lie in the range 20-80 nm with surface charge of -23 mV and also offering extremely fair biocompatibility.. The biocompatibility of the prepared nanocarriers was judged by BSA adsorption test and haemolysis assay. The in vitro release kinetics of the insulin loaded nanoparticles was studied in phosphate buffer saline (PBS) solution, and the influence of various factors such as pH, temperature and simulated physiological fluids was studied on the controlled release of insulin.
本研究的目的是制备和表征生物聚合物纳米载体，并评估其在胰岛素口服给药方面的适用性。使用卵清蛋白生物聚合物制备纳米颗粒，并用傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、zeta电位、动态光散射（DLS）和细胞毒性对其进行进一步表征。通过表征研究发现，纳米颗粒的尺寸在20-80nm范围内，表面电荷为-23mV，并且具有非常良好的生物相容性。通过牛血清白蛋白吸附试验和溶血试验来判断所制备纳米载体的生物相容性。在磷酸盐缓冲盐水（PBS）溶液中研究了负载胰岛素的纳米颗粒的体外释放动力学，并研究了pH、温度和模拟生理流体等各种因素对胰岛素控释的影响。
