Kodama M, Kamioka Y, Nakayama T, Nagata C, Morooka N, Ueno Y
Toxicol Lett. 1987 Jul;37(2):149-56. doi: 10.1016/0378-4274(87)90151-2.
Among several hydroxylated metabolites of emodin, a fungal anthraquinone and constituent of rhubarb, 2-hydroxyemodin was a direct-acting mutagen showing a large electron-spin resonance (ESR) signal in the presence of DNA, especially at alkaline pH. Coupled with generation of free radical, hydrogen peroxide but not superoxide was formed. The active oxygen produced from 2-hydroxyemodin induced strand breaks in phi X 174 replicative form I DNA (supercoiled covalently closed circular duplex DNA). These results suggest a possible role of active oxygen in the process of mutagenesis.
在大黄的成分之一真菌蒽醌大黄素的几种羟基化代谢产物中，2-羟基大黄素是一种直接作用的诱变剂，在有DNA存在时，尤其是在碱性pH条件下，会显示出较大的电子自旋共振（ESR）信号。伴随着自由基的产生，形成的是过氧化氢而不是超氧化物。2-羟基大黄素产生的活性氧可诱导φX 174复制型I DNA（超螺旋共价闭合环状双链DNA）发生链断裂。这些结果表明活性氧在诱变过程中可能发挥作用。
