姜黄素对脓毒症大鼠肺组织的抗氧化和抗炎作用。

Antioxidant and ntiinflammatory efficacy of curcumin on lung tissue in rats with sepsis.

机构信息

Faculty of Health Sciences, Department of Nutrition and Dietetics, Karabük University, Karabük 78000, Turkey.

Faculty of Health Sciences, Department of Nutrition and Dietetics, Gazi University, Ankara 06500, Turkey.

出版信息

J Tradit Chin Med. 2020 Oct;40(5):820-826. doi: 10.19852/j.cnki.jtcm.2020.05.012.

DOI:10.19852/j.cnki.jtcm.2020.05.012

PMID:33000583

Abstract

OBJECTIVE

To detect antioxidant and antiinflammatory efficacy of Curcumin (Cur) on lung tissue in rats with sepsis.

METHODS

Totally 32 rats were divided into 4 groups; the rats in Group 1 (control group) had abdominal incision under sterile conditions following anesthesia and the abdomen was sutured. Abdominal incision was performed in the rats in Group 2 (Cur group) under sterile conditions following anesthesia and the abdomen was closed. Cur was given to this group after dissolving within dimethylsulphoxide as 100 mg/kg through oral gavage and started for 3 d before surgical procedure. Group 3 (CLP group) had caecal ligation and punction (CLP) under sterile conditions to create sepsis following anesthesia and the abdomen was sutured. CLP was performed in the rats in Group 4 (CLP + Cur group) under sterile conditions following anesthesia to create a sepsis model and the abdomen was closed. Cur was also given to this group after dissolving within dimethylsulphoxide as 100 mg/kg through oral gavage and started for 3 d before surgical procedure. All the rats were sacrificed through blood aspiration from the heart under sterile conditions following anesthesia and lung tissues were removed after 24 h following the surgical procedures. The tissue samples were homogenizated for biochemical analyses; and malondialdehyde (MDA), nitric okxide (NO), myeloperoxidase (MPO), superoxidedysmutase (SOD) nd catalase (CAT) were analyzed through spectrophotometric method, immunhistochemical iNOS staining was performed to assess the inflammation; and histopathological differences between the groups were evaluated.

RESULTS

A statistically significant decrease was detected in the CLP + Cur group when compared with the CLP group of which Cur was not given in terms of MDA, MPO and NO levels (P < 0.05) whereas a statistically significant elevation was fpund in the CLP + Cur group when compared with the CLP group in terms of SOD and CAT levels (P < 0.05).

CONCLUSION

The study outcomes revealed that supplementation of Cur presents an antioxidant effect by reducing the free radical level and increasing the antioxidant enzyme levels; and an antiinflammatory effect by reducing iNOS level.

摘要

目的

检测姜黄素（Cur）对脓毒症大鼠肺组织的抗氧化和抗炎作用。

方法

32 只大鼠随机分为 4 组；第 1 组（对照组）大鼠在无菌条件下进行腹部切开，麻醉后缝合腹部。第 2 组（Cur 组）大鼠在无菌条件下进行腹部切开，麻醉后关闭腹部。该组大鼠在手术前 3 天通过口服灌胃溶解于二甲基亚砜中给予 100mg/kg 的 Cur。第 3 组（CLP 组）大鼠在无菌条件下进行盲肠结扎和穿孔（CLP）以创建脓毒症，麻醉后缝合腹部。第 4 组（CLP+Cur 组）大鼠在无菌条件下进行 CLP 以创建脓毒症模型，麻醉后关闭腹部。该组大鼠在手术前 3 天通过口服灌胃溶解于二甲基亚砜中给予 100mg/kg 的 Cur。所有大鼠在无菌条件下通过心脏采血麻醉后处死，手术 24 小时后取出肺组织。组织样本进行匀浆化用于生化分析；通过分光光度法分析丙二醛（MDA）、一氧化氮（NO）、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT），免疫组织化学 iNOS 染色用于评估炎症；并评估各组之间的组织病理学差异。