Liu Y-F, Yang C-W, Liu H, Sui S-G, Li X-D
Xiang-Dong Li, Emergency center, The Second Hospital of Dalian Medical University, NO.467 Zhongshan Road, ShaHekou District, Dalian, Liaoning 116023, China. E-mail:
J Nutr Health Aging. 2017;21(3):307-313. doi: 10.1007/s12603-016-0722-1.
The present study investigates curcumin effect against sepsis-induced chronic lung injury (CLI) of male albino rats. Rats were grouped into four groups such rats undergoing a sham cecal ligature puncture (CLP), rats undergoing CLP, rats undergoing CLP and treated with saline and rats undergoing CLP and treated with curcumin (100 mg/kg bwt). After 45 days of treatment, bronchoalveolar fluid (BALF), blood and lung tissues were collected from the each animal. The total protein content, wet and dry (W/D) weight of lung tissues and some inflammatory cells in the BALF were measured. Histopathological analysis was carried out to investigate the alteration of the cellular architecture of lung tissues. Lipid peroxidation malondialdehyde (MDA), superoxide dismutase (SOD) and myeloperoxidase (MPO) levels were determined. Cytokines such as interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-a) and macrophage inhibitory factor (MIF) were measured in the BALF. Curcumin administration significantly reduced CLP-induced inflammation and pulmonary edema. Curcumin treatment is significantly reduced MPO activity, and inflammatory cell accumulation in the BALF and also protein level, MDA, SOD, and W/D ratio were significantly reduced in the lung tissues. Also, curcumin reduced the expression of IL-A, TNF-a and MIF levels in the lung tissues. Histopathological study revealed the significant reduction of CLP-induced CLI in the curcumin-treated male albino rats. Taking all these data together, it is concluded that curcumin can act as a suitable therapeutic agent against CLP-induced CLI of male albino rats.
本研究调查了姜黄素对雄性白化病大鼠脓毒症诱导的慢性肺损伤（CLI）的影响。将大鼠分为四组：接受假盲肠结扎穿刺（CLP）的大鼠、接受CLP的大鼠、接受CLP并给予生理盐水治疗的大鼠以及接受CLP并给予姜黄素（100mg/kg体重）治疗的大鼠。治疗45天后，从每只动物收集支气管肺泡灌洗液（BALF）、血液和肺组织。测量肺组织的总蛋白含量、湿重和干重（W/D）以及BALF中的一些炎性细胞。进行组织病理学分析以研究肺组织细胞结构的改变。测定脂质过氧化丙二醛（MDA）、超氧化物歧化酶（SOD）和髓过氧化物酶（MPO）水平。在BALF中测量细胞因子，如白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）和巨噬细胞抑制因子（MIF）。给予姜黄素可显著减轻CLP诱导的炎症和肺水肿。姜黄素治疗可显著降低MPO活性以及BALF中的炎性细胞积聚，并且肺组织中的蛋白水平、MDA、SOD和W/D比值也显著降低。此外，姜黄素降低了肺组织中IL-8、TNF-α和MIF水平的表达。组织病理学研究显示，在接受姜黄素治疗的雄性白化病大鼠中，CLP诱导的CLI显著减轻。综合所有这些数据，可以得出结论，姜黄素可作为治疗雄性白化病大鼠CLP诱导的CLI的合适治疗剂。
