Department of Pharmacology, CMR College of Pharmacy, Hyderabad, Telangana State, India.
Department of Biotechnology, IIT-Hyderabad, Sangareddy, Telangana State, India.
J Basic Clin Physiol Pharmacol. 2020 Oct 1;32(2):79-87. doi: 10.1515/jbcpp-2019-0362.
The main objective of the present study is to evaluate the mitigative effect of hydroalcoholic extract of fruits against sodium fluoride (NaF) induced hepatotoxicity.
In this study, Wistar male albino rats were randomly divided into five groups of six rats each. Group I and II served as normal and toxic controls. Group III as plant control received extract at a dose of 400 mg/kg b. wt, and Groups IV and V as treatment groups received extract at a dose 200 and 400 mg/kg b. wt, for 30 days. All groups except Groups I and III received 100 ppm of NaF through drinking water. After completion of the study, blood collected for the estimation of liver blood serum biomarkers such as aspartate aminotransferases (AST), alanine aminotransferases (ALT), alkaline Phosphatase (ALP), direct and total bilirubin, total protein and albumin. The liver tissue homogenate was for estimation of lipid peroxidation, catalase, and reduced glutathione levels.
The results showed that NaF intoxication caused elevation of liver blood serum levels and lipid peroxidation; decreased levels of serum total protein, albumin and liver reduced glutathione, and catalase observed. The treatment groups showed decreased elevated serum biomarkers (ALT, AST, and ALP), liver lipid peroxidation and increased serum total protein and albumin, liver reduced glutathione and catalase levels in a dose-dependent manner. Histopathological studies also further strongly supported for mitigative effects of the plant.
In conclusion, our findings of the study indicated that fruits were a potential drug candidate in the treatment of NaF induced hepatotoxicity.
本研究的主要目的是评估 果实的水醇提取物对氟酸钠（NaF）诱导的肝毒性的缓解作用。
在这项研究中，Wistar 雄性白化大鼠随机分为五组，每组六只。第 I 组和第 II 组作为正常和毒性对照组。第 III 组作为植物对照组，给予 400mg/kg 体重的提取物；第 IV 组和第 V 组作为治疗组，分别给予 200 和 400mg/kg 体重的提取物，连续 30 天。除第 I 组和第 III 组外，所有组均通过饮用水摄入 100ppm 的 NaF。研究完成后，采集血液用于估计血清肝生物标志物，如天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、直接和总胆红素、总蛋白和白蛋白。肝组织匀浆用于估计脂质过氧化、过氧化氢酶和还原型谷胱甘肽水平。
结果表明，NaF 中毒导致血清水平升高和脂质过氧化；血清总蛋白、白蛋白和肝还原型谷胱甘肽以及过氧化氢酶水平降低。治疗组表现出血清生物标志物（ALT、AST 和 ALP）升高的降低，肝脂质过氧化和血清总蛋白、白蛋白、肝还原型谷胱甘肽和过氧化氢酶水平的增加呈剂量依赖性。组织病理学研究也进一步强有力地支持了植物的缓解作用。
总之，我们的研究结果表明， 果实是治疗 NaF 诱导的肝毒性的潜在候选药物。
