Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, China.
PLoS One. 2020 Oct 1;15(10):e0239736. doi: 10.1371/journal.pone.0239736. eCollection 2020.
The identification of anammox bacteria is mostly relied on PCR with various marker genes. However, the community composition revealed by different marker genes and whether the marker genes influence the resulted community composition remain unclear. We compared the community structure of anammox bacteria in enriched and natural environments revealed by 16S rRNA and functional genes (hzo, hzsA and hzsB) from public database and published papers. The genus of Ca. Scalindua showed the lowest similarities with other genera, especially for the hzsA gene (66.9%-68.6%). The 16S rRNA gene is the most commonly used marker gene in natural habitats with 151 out 221 papers in total. The anammox bacterial community composition is distributed according to the source of habitat regardless the use of various marker genes. The role of marker gene is limited with explanatory of 5.4% for variance of community composition, versus 20.5% of habitat. The effect of marker gene is mainly acted on freshwater habitat, which shows significant different community composition revealed by 16S rRNA and hzo, with Ca. Brocadia and Ca. Jettenia as dominant genus, respectively.
氨氧化菌的鉴定主要依赖于带有各种标记基因的聚合酶链式反应（PCR）。然而，不同标记基因所揭示的群落组成以及标记基因是否会影响所得群落组成尚不清楚。我们比较了公共数据库和已发表论文中 16S rRNA 和功能基因（hzo、hzsA 和 hzsB）揭示的富集和自然环境中氨氧化菌的群落结构。Ca. Scalindua 属与其他属的相似性最低，特别是 hzsA 基因（66.9%-68.6%）。16S rRNA 基因是自然生境中最常用的标记基因，在总共 221 篇论文中有 151 篇使用了该基因。氨氧化菌群落组成根据栖息地来源分布，而不考虑各种标记基因的使用。标记基因的作用是有限的，对群落组成的解释方差仅为 5.4%，而栖息地的解释方差为 20.5%。标记基因的作用主要作用于淡水生境，16S rRNA 和 hzo 揭示的群落组成存在显著差异，分别以 Ca. Brocadia 和 Ca. Jettenia 为优势属。
