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撤回:KDM4A 通过 DLX5 介导 Myc 的表达,通过 Wnt/β-catenin 信号通路促进非小细胞肺癌的生长。

RETRACTED: KDM4A promotes the growth of non-small cell lung cancer by mediating the expression of Myc via DLX5 through the Wnt/β-catenin signaling pathway.

机构信息

Department of General Oncotherapy, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai 264200, Shandong, PR China.

Department of Pathology, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai 264200, Shandong, PR China.

出版信息

Life Sci. 2020 Dec 1;262:118508. doi: 10.1016/j.lfs.2020.118508. Epub 2020 Sep 28.

DOI:10.1016/j.lfs.2020.118508
PMID:33002480
Abstract

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the authors as they “found major problems in the data and conclusions through their later research”. 1. When the authors performed flow cytometry to detect apoptosis, the Annexin-V-coupled fluorophore they used was Fluor 647 (as described in the Methods section), which was incorrectly labelled as the Annexin-V-coupled fluorophore as FITC in their Figures (Fig. 5E, 7C and Fig. S1D). The excitation wavelengths of Alexa Fluor 647 (594/633 nm) are different from that of FITC (490 nm/520 nm), this mistake would lead to unreliability of their data. 2. The authors discovered a major error during the traceability of the antibodies used in the experiments. The primary antibody they used to detect KDM4A was actually a primary antibody for KDM6B, as evidenced by the western blots. KDM6B is a 177-kDa protein (consistent with the kDa shown in Fig. 1K and Fig. 2B), while KDM4A is a 150-kDa protein. 3. Lastly, the authors carelessly mislabeled KDM4A as KDM4B in Fig. 7. The authors and the Editors believe that the conclusions of the paper are not dependable, so we have decided to retract the paper. Apologies are offered to readers of the journal that this was not detected during the submission process.

摘要

本文已被撤回

请参阅 Elsevier 关于撤稿政策(https://www.elsevier.com/about/our-business/policies/article-withdrawal)。应作者要求,本文已被撤回,因为他们“在后续研究中发现数据和结论存在重大问题”。1. 当作者进行流式细胞术检测细胞凋亡时,他们使用的 Annexin-V 偶联荧光染料是 Fluor 647(如方法部分所述),但在他们的图(图 5E、7C 和图 S1D)中错误地标为 Annexin-V 偶联荧光染料 FITC。Alexa Fluor 647(594/633nm)的激发波长与 FITC(490nm/520nm)不同,这个错误会导致他们的数据不可靠。2. 作者在实验中使用的抗体追踪过程中发现了一个重大错误。他们用于检测 KDM4A 的一抗实际上是 KDM6B 的一抗,这可以从 Western blot 中得到证明。KDM6B 是一种 177kDa 的蛋白质(与图 1K 和图 2B 中所示的 kDa 一致),而 KDM4A 是一种 150kDa 的蛋白质。3. 最后,作者在图 7 中粗心地将 KDM4A 误标为 KDM4B。作者和编辑认为,该论文的结论不可靠,因此我们决定撤回该论文。向本刊读者表示歉意,在提交过程中未能发现这一点。

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