口腔鳞状细胞癌（OSCC）患者中的低密度粒细胞（LDGs）与正常密度粒细胞（NDGs）
LDGs versus NDGs in patients with oral squamous cell carcinoma (OSCC).
作者信息
Garley Marzena, Jabłońska Ewa, Dziemiańczyk-Pakieła Dorota, Miltyk Wojciech, Ratajczak-Wrona Wioletta, Borys Jan, Moniuszko Marcin, Grubczak Kamil
机构信息
Department of Immunology Medical, University of Bialystok, J. Waszyngtona 15A, 15-269 Bialystok, Poland.
Department of Immunology Medical, University of Bialystok, J. Waszyngtona 15A, 15-269 Bialystok, Poland.
出版信息
Cytokine. 2021 Jan;137:155311. doi: 10.1016/j.cyto.2020.155311. Epub 2020 Sep 29.
BACKGROUND
The significant role played by neutrophils in cancer biology is indisputable; yet, their subpopulations may exhibit a contrasting role. The phenomenon of polarization of neutrophils and signaling modulators in the course of a neoplastic process has gained increased attention in recent times. The present study's objective was to quantitatively assess low-density neutrophils (LDNs) and normal-density neutrophils (NDNs) populations including IL-17 expression in confrontation with Th17 lymphocytes in patients with oral squamous cell carcinoma (OSCC). The neutrophil to lymphocyte ratio (NLR) biomarker value was determined. Besides, the influence of rhIL-17 on the proliferation level of the squamous cell carcinoma (SCC) malignant line cells was tested.
METHODS
Leukocytes were isolated in the density gradient and the CD16+ population was magnetically sorted. The percentages of neutrophil subpopulations, lymphocyte Th17, and IL-17 expression in the studied cells were determined on a flow cytometer. Squamous cell carcinoma proliferation was assessed with the MTT test.
RESULT
The existence of two populations of human neutrophils was determined: LDNs and NDNs. A higher percentage of LDNs and Th17 was observed with the concomitant lower percentage of NDNs in patients with OSCC as compared with the control group. NLR was elevated in patients with cancer. The highest IL-17 expression was obtained in the LDNs population in these patients. However, no influence of IL-17 on SCC proliferation could be determined.
CONCLUSION
The present study demonstrated a strong relationship between IL-17 concentration and the count of LDNs or Th17 in the course of OSCC, which may serve as a reference point for new therapies. Moreover, the obtained LDNs/NDNs and NLR values in patients with cancer prove their usefulness in diagnostic and prognostic in patients with OSCC.
背景
中性粒细胞在癌症生物学中所起的重要作用是无可争议的；然而，它们的亚群可能表现出相反的作用。近年来，中性粒细胞极化现象以及肿瘤形成过程中的信号调节因子越来越受到关注。本研究的目的是定量评估口腔鳞状细胞癌（OSCC）患者中低密度中性粒细胞（LDNs）和正常密度中性粒细胞（NDNs）群体，包括与Th17淋巴细胞对抗时的白细胞介素-17（IL-17）表达。测定中性粒细胞与淋巴细胞比值（NLR）生物标志物值。此外，还测试了重组人白细胞介素-17（rhIL-17）对鳞状细胞癌（SCC）恶性系细胞增殖水平的影响。
方法
通过密度梯度分离白细胞，并对CD16+群体进行磁性分选。在流式细胞仪上测定所研究细胞中中性粒细胞亚群、淋巴细胞Th17和IL-17表达的百分比。用MTT试验评估鳞状细胞癌的增殖情况。
结果
确定了人类中性粒细胞的两个群体：LDNs和NDNs。与对照组相比，OSCC患者中LDNs和Th17的百分比更高，而NDNs的百分比更低。癌症患者的NLR升高。这些患者中LDNs群体的IL-17表达最高。然而，未确定IL-17对SCC增殖有影响。
结论
本研究表明在OSCC过程中IL-17浓度与LDNs或Th17计数之间存在密切关系，这可能为新疗法提供参考点。此外，癌症患者获得的LDNs/NDNs和NLR值证明它们在OSCC患者的诊断和预后中有用。
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