Dental and Life Science Institute, Pusan National University, Yangsan 50612, Korea.
Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea.
Int J Mol Sci. 2020 Sep 29;21(19):7209. doi: 10.3390/ijms21197209.
Gomisin N (GN) is lignin derived from that has been reported to exhibit hepato-protective, anti-cancer, and anti-inflammatory effects. However, its role in whole-body energetic homeostasis remains unclear. In this study, we employed as a diet-induced obese model to elucidate the effects of GN on lipid and glucose metabolism by measuring climbing activity, triglyceride levels, and lifespan under a rearing condition of a high-fat diet (HFD) containing 20% coconut oil, with or without GN. Constant exposure of flies to an HFD resulted in increased body weight and decreased climbing activity, along with a shortened life span. Importantly, the administration of GN to HFD groups lowered their body weight and induced a specific upregulation of lipid storage droplet () and hormone-sensitive lipase (), in addition to improved lifespan. Importantly, GN in HFD groups appeared to downregulate heat shock protein Hsp90 family member (), a key regulator of the endoplasmic reticulum stress response, which may also contribute to improved life span in the presence of GN. Taken together, these in vivo findings suggest that GN could serve as a useful agent for the prevention and treatment of obesity.
戈米辛 N（GN）是一种来源于 的木质素，已被报道具有肝保护、抗癌和抗炎作用。然而，其在全身能量稳态中的作用尚不清楚。在这项研究中，我们使用 作为饮食诱导肥胖模型，通过测量攀爬活动、甘油三酯水平和在高脂肪饮食（HFD）饲养条件下的寿命来阐明 GN 对脂质和葡萄糖代谢的影响，HFD 中含有 20%的椰子油，有无 GN 。苍蝇持续暴露于 HFD 会导致体重增加和攀爬活动减少，寿命缩短。重要的是，GN 的给药可降低 HFD 组的体重，并诱导脂质储存滴（）和激素敏感脂肪酶（）的特异性上调，此外还可延长寿命。重要的是，HFD 组中的 GN 似乎下调了热休克蛋白 90 家族成员（），这是内质网应激反应的关键调节剂，这也可能有助于在存在 GN 的情况下延长寿命。总之，这些体内发现表明 GN 可以作为预防和治疗肥胖的有用药物。
