Ordóñez N G, Brooks T, Thompson S, Batsakis J G
Am J Surg Pathol. 1987 Jul;11(7):543-50. doi: 10.1097/00000478-198707000-00006.
Lymphatic and blood vessel invasion is one of the most important diagnostic and prognostic parameters used by pathologists in the evaluation of neoplastic conditions. Although a variety of tissue artifacts can make the recognition of vascular, capillary, and lymphatic permeation by tumor cells extremely difficult, recent immunohistochemical studies have employed a variety of tissue markers that appear to have great value in establishing the diagnosis of lymphovascular involvement. In the present study, we applied an immunoperoxidase staining technique to previously stained microscopic tissue sections using Ulex europaeus agglutinin I lectin, an endothelial marker that can be used in paraffin-embedded tissues. Our results indicate that this technique can be successfully applied on microscopic slides previously stained with a variety of histochemical stains. It can also be used in those cases in which paraffin blocks are not available or the area in question is absent from the recut tissue sections.
淋巴管和血管侵犯是病理学家在评估肿瘤疾病时使用的最重要的诊断和预后参数之一。尽管多种组织假象会使识别肿瘤细胞的血管、毛细血管和淋巴管浸润极其困难，但最近的免疫组织化学研究采用了多种组织标志物，这些标志物在确立淋巴管受累的诊断方面似乎具有很大价值。在本研究中，我们使用欧洲荆豆凝集素I（一种可用于石蜡包埋组织的内皮标志物）对先前染色的显微组织切片应用免疫过氧化物酶染色技术。我们的结果表明，该技术可成功应用于先前用多种组织化学染色剂染色的显微玻片。它也可用于那些没有石蜡块或重新切片的组织切片中不存在相关区域的病例。
