Department of Mechanical Engineering and Materials Science, Duke University, Hudson Hall, Durham, North Carolina 27708.
Division of Rheumatology and Immunology, Duke University Medical Center, 508 Fulton St, Durham, North Carolina 27707.
Biointerphases. 2020 Oct 1;15(5):051006. doi: 10.1116/6.0000439.
There has been much recent interest in the protein "corona," the nonspecific adsorption of proteins on the surface of nanoparticles used in biological applications. This research investigates an analogous DNA corona. We find that particles (200 nm and 1 μm) incubated with DNA form a DNA corona, with a higher concentration of DNA adsorbed on the surface of cationic nanoparticles. With protein present, a combined DNA and protein corona is formed although DNA in solution displaces protein from the nanoparticle surface. Displacement of protein from the nanoparticle surface is dependent on the concentration of DNA in solution and was also observed for planar surfaces. Overall, we expect this investigation of the DNA corona to be important for nanomedicine applications, as well as disease states, especially systemic lupus erythematosus, in which biological particles with bound DNA are important mediators of inflammation and thrombosis.
最近人们对蛋白质“冠”（corona）产生了浓厚的兴趣，蛋白质在用于生物应用的纳米粒子表面的非特异性吸附。本研究调查了类似的 DNA 冠。我们发现，与 DNA 孵育的颗粒（200nm 和 1μm）形成了 DNA 冠，带有更高浓度的 DNA 吸附在阳离子纳米颗粒的表面。当存在蛋白质时，尽管溶液中的 DNA 会从纳米粒子表面置换蛋白质，但会形成一个结合 DNA 和蛋白质的冠。从纳米粒子表面置换蛋白质取决于溶液中 DNA 的浓度，在平面表面也观察到了这种情况。总的来说，我们预计对 DNA 冠的这种研究对纳米医学应用以及疾病状态（尤其是红斑狼疮）很重要，在这些疾病状态中，带有结合 DNA 的生物颗粒是炎症和血栓形成的重要介质。
