Degl'Innocenti Andrea, Braccia Clarissa, Genchi Giada Graziana, di Leo Nicoletta, Leoncino Luca, Catalano Federico, Armirotti Andrea, Ciofani Gianni
Smart Bio-Interfaces, Center for Materials Interfaces, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, Pontedera, Pisa 56025, Italy.
Department of Medical Biotechnologies, Polyclinic Hospital Santa Maria alle Scotte, Università degli Studi di Siena, Viale Mario Bracci 2, Siena 53100, Italy.
ACS Omega. 2024 Jun 27;9(27):29226-29233. doi: 10.1021/acsomega.3c09715. eCollection 2024 Jul 9.
Oxidative stress is a widespread causative agent of disease. Together with its general relevance for biomedicine, such a dynamic is recognizably detrimental to space exploration. Among other solutions, cerium oxide nanoparticles (or , NC) display a long-lasting, self-renewable antioxidant activity. In a previous experiment, we evaluated oxidative imbalance in rat myoblasts in space, aboard the International Space Station, and unveiled possible protective effects from NC through RNA sequencing. Here, we focus on the myoblast response to NC on land by means of proteomics, defining a list of proteins that putatively react to NC and confirming nucleosomes/histones as likely mediators of its molecular action. The proteomics data set we present here and its counterpart from the space study share four factors. These are coherently either up- () or down-regulated (, , ) upon NC exposure.
氧化应激是一种广泛存在的致病因素。鉴于其在生物医学中的普遍相关性，这种动态变化对太空探索显然是有害的。在其他解决方案中，氧化铈纳米颗粒（或CeO₂ NPs，NC）具有持久的、可自我更新的抗氧化活性。在之前的一项实验中，我们在国际空间站上评估了太空环境中大鼠成肌细胞的氧化失衡情况，并通过RNA测序揭示了NC可能具有的保护作用。在这里，我们通过蛋白质组学研究陆地环境下成肌细胞对NC的反应，确定了一组可能对NC产生反应的蛋白质，并证实核小体/组蛋白可能是其分子作用的介质。我们在此展示的蛋白质组学数据集及其在太空研究中的对应数据集共有四个因素。在NC暴露后，这些因素一致地出现上调（+）或下调（-，-，-）。
