miR-30a 在卵巢癌中的预后价值及潜在通路信号的生物信息学分析。

Bioinformatics analysis of prognostic value and prospective pathway signal of miR-30a in ovarian cancer.

机构信息

Guangzhou University of Chinese Medicine, No.232, Waihuandong Road, University Town, Panyu District, Guangzhou, 510006, Guangdong, China.

Department of Gynaecological Oncology, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, 510095, Guangdong Province, China.

出版信息

J Ovarian Res. 2020 Oct 1;13(1):120. doi: 10.1186/s13048-020-00722-8.

DOI:10.1186/s13048-020-00722-8

PMID:33004058

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7532093/

Abstract

OBJECTIVE

MicroRNAs (MiRNAs) is thought to play a critical role in the initiation and progress of ovarian cancer (OC). Although miRNAs has been widely recognized in ovarian cancer, the role of hsa-miR-30a-5p (miR-30a) in OC has not been fully elucidated.

METHODS

Three mRNA datasets of normal ovarian tissue and OC, GSE18520,GSE14407 and GSE36668, were downloaded from Gene Expression Omnibus (GEO) to find the differentially expressed gene (DEG). Then the target genes of hsa-miR-30a-5p were predicted by miRWALK3.0 and TargetScan. Then, the gene overlap between DEG and the predicted target genes of miR-30a in OC was analyzed by Gene Ontology (GO) enrichment analysis. Protein-protein interaction (PPI) network was conducted by STRING and Cytoscape, and the effect of HUB gene on the outcome of OC was analyzed.

RESULTS

A common pattern of up-regulation of miR-30a in OC was found. A total of 225 DEG, were identified, both OC-related and miR-30a-related. Many DEG are enriched in the interactions of intracellular matrix tissue, ion binding and biological process regulation. Among the 10 major Hub genes analyzed by PPI, five Hub genes were significantly related to the overall poor survival of OC patients, in which the low expression of ESR1,MAPK10, Tp53 and the high expression of YKT,NSF were related to poor prognosis of OC.

CONCLUSION

Our results indicate that miR-30a is of significance for the biological progress of OC.

摘要

目的

微小 RNA（miRNA）被认为在卵巢癌（OC）的发生和进展中起着关键作用。尽管 miRNA 在卵巢癌中已得到广泛认可，但 hsa-miR-30a-5p（miR-30a）在 OC 中的作用尚未完全阐明。

方法

从基因表达综合数据库（GEO）下载了三个正常卵巢组织和 OC 的 mRNA 数据集，GSE18520、GSE14407 和 GSE36668，以寻找差异表达基因（DEG）。然后通过 miRWALK3.0 和 TargetScan 预测 hsa-miR-30a-5p 的靶基因。然后，通过基因本体论（GO）富集分析分析 OC 中 DEG 与 miR-30a 的预测靶基因之间的基因重叠。通过 STRING 和 Cytoscape 进行蛋白质-蛋白质相互作用（PPI）网络构建，并分析 HUB 基因对 OC 结局的影响。

结果

发现 OC 中 miR-30a 的上调模式具有共同性。共鉴定出 225 个 DEG，包括 OC 相关和 miR-30a 相关的 DEG。许多 DEG 富集于细胞内基质组织、离子结合和生物过程调节的相互作用中。在 PPI 分析的 10 个主要 HUB 基因中，有 5 个 HUB 基因与 OC 患者总体预后不良显著相关，其中 ESR1、MAPK10、Tp53 表达水平低和 YKT、NSF 表达水平高与 OC 的不良预后相关。