Materials and Analysis Department, Hyogo Prefectural Institute of Technology, 3-1-12, Yukihira, Suma, Kobe, 654-0037, Japan.
Graduate School of Engineering, University of Hyogo, 2167, Shosha, Himeji, 671-2201, Japan.
Sci Rep. 2020 Oct 1;10(1):16363. doi: 10.1038/s41598-020-73485-3.
To manage health conditions of farmed fish and other living creatures, a simple method to measure bioelectric signals of the creatures in seawater is expected. A novel method to measure bioelectric signals by utilizing the conductivity of seawater surrounding the entire body of a fish is proposed. As for the proposed method, a needle-type internal electrode is inserted into the fish's muscle at a certain measurement point, and an external electrode is sunk in seawater. The internal electrode is isolated from the seawater by virtue of being inserted in the fish. Bioelectric signals generated between the external and internal electrodes are then measured. By sharing the external electrode with the internal electrode, it is possible to measure bioelectric signals with half the number of bioelectrodes used by conventional methods. To demonstrate the practicality of the proposed method, two internal electrodes were inserted into different parts (above the gills and near the tail) of three fish (Parajulis poecilepterus, ca. 20 cm fork length) kept in a tank. The proposed method obtained reliable bioelectric signals corresponding to electrocardiograms (ECGs) and electromyograms (EMGs) from each part of the individual fish.
为了管理养殖鱼类和其他生物的健康状况，人们期望有一种简单的方法来测量其在海水中的生物电信号。本文提出了一种利用环绕鱼类全身的海水导电性来测量生物电信号的新方法。对于所提出的方法，将针型内电极插入鱼的肌肉中的某个测量点，并将外电极沉入海水中。内部电极通过插入鱼体内与海水隔离，然后测量外部和内部电极之间产生的生物电信号。通过与内部电极共享外部电极，可以用传统方法一半的生物电极数量来测量生物电信号。为了验证该方法的实用性，将两个内电极插入了三条鱼（Parajulis poecilepterus，约 20 厘米叉长）的不同部位（鳃上和尾部附近），这些鱼被饲养在一个水箱中。该方法从个体鱼的各个部位获得了可靠的生物电信号，这些信号对应心电图（ECG）和肌电图（EMG）。
