Jin Jingjing, Liu Dan, Qi Yongzhi, Ma Jun, Zhen Wenchao
College of Plant Protection, Hebei Agricultural University, Baoding, China.
State Key Laboratory of North China Crop Improvement and Regulation, Baoding, China.
Front Genet. 2020 Aug 28;11:1012. doi: 10.3389/fgene.2020.01012. eCollection 2020.
Flag leaves, plant height (PH), and spike-related traits are key determinants contributing to yield potential in wheat. In this study, we developed a recombinant inbred line (RIL) population with 94 lines derived from the cross between 'AS985472' and 'Sumai 3.' A genetic map spanned 3553.69 cM in length were constructed using 1978 DArT markers. Severn traits including flag leaf width (FLW), flag leaf length (FLL), PH, anthesis date (AD), spike length (SL), spikelet number spike (SNS), and spike density (SD) were evaluated against this RIL population under three different environments. Combined phenotypic data and genetic map, we identified quantitative trait loci (QTL) for each trait. A total of four major and stably expressed QTLs for FLW, PH, and SD were detected on chromosomes 2D and 4B. Of them, the major QTLs individually explained 10.10 - 30.68% of the phenotypic variation. QTLs with pleiotropic effects were identified on chromosomes 4A and 6D as well. Furthermore, the genetic relationships between seven yield-related traits were detected and discussed. A few genes related to leaf growth and development at the interval of a major locus for FLW on chromosome 2D were predicated. Overall, the present study provided useful information for understanding the genetic basis of yield-related traits and will be useful for marker-assisted selection in wheat breeding.
旗叶、株高（PH）和穗部相关性状是决定小麦产量潜力的关键因素。在本研究中，我们构建了一个包含94个株系的重组自交系（RIL）群体，该群体源于‘AS985472’和‘苏麦3号’的杂交。利用1978个DArT标记构建了一条长度为3553.69 cM的遗传图谱。针对该RIL群体，在三种不同环境下对包括旗叶宽度（FLW）、旗叶长度（FLL）、株高、抽穗期（AD）、穗长（SL）、小穗数（SNS）和穗密度（SD）在内的七个性状进行了评估。结合表型数据和遗传图谱，我们鉴定了每个性状的数量性状位点（QTL）。在2D和4B染色体上共检测到四个与FLW、PH和SD相关的主要且稳定表达的QTL。其中，这些主要QTL分别解释了10.10 - 30.68%的表型变异。在4A和6D染色体上也鉴定到了具有多效性的QTL。此外，还检测并讨论了七个产量相关性状之间的遗传关系。预测了位于2D染色体上一个与FLW主要位点区间相关的一些与叶片生长发育有关的基因。总体而言，本研究为理解产量相关性状的遗传基础提供了有用信息，并且将对小麦育种中的标记辅助选择有用。
