State Key Laboratory of Crop Biology/Key Laboratory of Crop Water Physiology and Drought-Tolerance Germplasm Improvement, Ministry of Agriculture/Group of Wheat Quality Breeding, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
Theor Appl Genet. 2018 May;131(5):1073-1090. doi: 10.1007/s00122-018-3059-9. Epub 2018 Feb 22.
Coincident regions on chromosome 4B for GW, on 5A for SD and TSS, and on 3A for SL and GNS were detected through an integration of a linkage analysis and a genome-wide association study (GWAS). In addition, six stable QTL clusters on chromosomes 2D, 3A, 4B, 5A and 6A were identified with high PVE% on a composite map. The panicle traits of wheat, such as grain number per spike and 1000-grain weight, are closely correlated with grain yield. Superior and effective alleles at loci related to panicles developments play a crucial role in the progress of molecular improvement in wheat yield breeding. Here, we revealed several notable allelic variations of seven panicle-related traits through an integration of genome-wide association mapping and a linkage analysis. The linkage analysis was performed using a recombinant inbred line (RIL) population (173 lines of F) with a high-density genetic map constructed with 90K SNP arrays, Diversity Arrays Technology (DArT) and simple sequence repeat (SSR) markers in five environments. Thirty-five additive quantitative trait loci (QTL) were discovered, including eleven stable QTLs on chromosomes 1A, 2D, 4B, 5B, 6B, and 6D. The marker interval between EX_C101685 and RAC875_C27536 on chromosome 4B exhibited pleiotropic effects for GW, SL, GNS, FSN, SSN, and TSS, with the phenotypic variation explained (PVE) ranging from 5.40 to 37.70%. In addition, an association analysis was conducted using a diverse panel of 205 elite wheat lines with a composite map (24,355 SNPs) based on the Illumina Infinium assay in four environments. A total of 73 significant marker-trait associations (MTAs) were detected for panicle traits, which were distributed across all wheat chromosomes except for 4D, 5D, and 6D. Consensus regions between RAC875_C27536_611 and Tdurum_contig4974_355 on chromosome 4B for GW in multiple environments, between QTSS5A.7-43 and BS00021805_51 on 5A for SD and TSS, and between QSD3A.2-164 and RAC875_c17479_359 on 3A for SL and GNS in multiple environments were detected through linkage analysis and a genome-wide association study (GWAS). In addition, six stable QTL clusters on chromosomes 2D, 3A, 4B, 5A, and 6A were identified with high PVE% on a composite map. This study provides potentially valuable information on the dissection of yield-component traits and valuable genetic alleles for molecular-design breeding or functional gene exploration.
通过连锁分析和全基因组关联研究（GWAS）的整合，在染色体 4B 上检测到与 GW 相关的重合区域、在染色体 5A 上与 SD 和 TSS 相关的重合区域、在染色体 3A 上与 SL 和 GNS 相关的重合区域。此外，在复合图谱上，在染色体 2D、3A、4B、5A 和 6A 上鉴定出六个具有高 PVE%的稳定 QTL 簇。小麦的穗部性状，如每穗粒数和千粒重，与产量密切相关。与穗部发育相关的位点的优良和有效等位基因在小麦产量育种的分子改良进展中起着至关重要的作用。在这里，我们通过全基因组关联作图和连锁分析的整合，揭示了七个穗相关性状的几个显著的等位基因变异。连锁分析使用重组自交系（RIL）群体（173 个 F 代系）进行，该群体使用 90K SNP 阵列、多样性阵列技术（DArT）和简单序列重复（SSR）标记在五个环境中构建了高密度遗传图谱。发现了 35 个加性数量性状位点（QTL），包括染色体 1A、2D、4B、5B、6B 和 6D 上的 11 个稳定 QTL。染色体 4B 上 EX_C101685 和 RAC875_C27536 之间的标记区间对 GW、SL、GNS、FSN、SSN 和 TSS 具有多效性效应，表型变异解释（PVE）范围为 5.40%至 37.70%。此外，在四个环境中，使用基于 Illumina Infinium 测定法的 205 个精选小麦系的综合图谱（24355 个 SNP）进行了关联分析。在穗部性状中检测到 73 个显著的标记-性状关联（MTA），分布在除 4D、5D 和 6D 之外的所有小麦染色体上。在多个环境中，在染色体 4B 上，RAC875_C27536_611 和 Tdurum_contig4974_355 之间、染色体 5A 上，QTSS5A.7-43 和 BS00021805_51 之间、染色体 3A 上，QSD3A.2-164 和 RAC875_c17479_359 之间，以及在多个环境中，在染色体 4B 上的 GW、染色体 5A 上的 SD 和 TSS、染色体 3A 上的 SL 和 GNS，通过连锁分析和全基因组关联研究（GWAS）进行了检测。此外，在复合图谱上，在染色体 2D、3A、4B、5A 和 6A 上鉴定出六个具有高 PVE%的稳定 QTL 簇。本研究为产量构成性状的剖析和分子设计育种或功能基因探索的有价值的遗传等位基因提供了有价值的信息。
