Wang Yuying, Zeng Zhankui, Li Jiachuang, Zhao Dehui, Zhao Yue, Peng Chen, Lan Caixia, Wang Chunping
College of Agronomy, Henan University of Science and Technology, Luoyang, 471000 Henan China.
The Shennong Laboratory, Zhengzhou, 450002 Henan China.
Mol Breed. 2023 Jul 31;43(8):64. doi: 10.1007/s11032-023-01401-4. eCollection 2023 Aug.
Wheat ( L.) is one of the most important cereal crops for ensuring food security worldwide. Identification of major quantitative trait loci (QTL) for spike-related traits is important for improvement of yield potential in wheat breeding. In this study, by using the wheat 55K single nucleotide polymorphism (SNP) array and diversity array technology (DArT), two recombinant inbred line populations derived from crosses avocet/chilero and avocet/huites were used to map QTL for kernel number per spike (KNS), total spikelet number per spike (TSS), fertile spikelet number per spike (FSS), and spike compactness (SC). Forty-two QTLs were identified on chromosomes 2A (4), 2B (3), 3A (2), 3B (7), 5A (11), 6A (4), 6B, and 7A (10), explaining 3.13-21.80% of the phenotypic variances. Twelve QTLs were detected in multi-environments on chromosomes 2A, 3B (2), 5A (4), 6A (3), 6B, and 7A, while four QTL clusters were detected on chromosomes 3A, 3B, 5A, and 7A. Two stable and new QTL clusters, and , were detected in the physical intervals of 547.49-590.46 Mb and 511.54-516.15 Mb, accounting for 7.53-14.78% and 7.01-20.66% of the phenotypic variances, respectively. High-confidence annotated genes for and were more highly expressed in spike development The results provide new QTL and molecular markers for marker-assisted breeding in wheat.
The online version contains supplementary material available at 10.1007/s11032-023-01401-4.
小麦（L.）是确保全球粮食安全的最重要谷类作物之一。鉴定与穗相关性状的主要数量性状位点（QTL）对于提高小麦育种的产量潜力很重要。在本研究中，利用小麦55K单核苷酸多态性（SNP）芯片和多样性阵列技术（DArT），使用两个由avocet/chilero和avocet/huites杂交衍生的重组自交系群体来定位每穗粒数（KNS）、每穗小穗总数（TSS）、每穗可育小穗数（FSS）和穗紧密度（SC）的QTL。在2A（4个）、2B（3个）、3A（2个）、3B（7个）、5A（11个）、6A（4个）、6B和7A（10个）染色体上鉴定出42个QTL，解释了3.13%-21.80%的表型变异。在2A、3B（2个）、5A（4个）、6A（3个）、6B和7A染色体的多个环境中检测到12个QTL，而在3A、3B、5A和7A染色体上检测到4个QTL簇。在547.49-590.46 Mb和511.54-516.15 Mb的物理区间内检测到两个稳定的新QTL簇，分别占表型变异的7.53%-14.78%和7.01%-20.66%。与 和 相关的高可信度注释基因在穗发育过程中表达更高。这些结果为小麦的分子标记辅助育种提供了新的QTL和分子标记。
在线版本包含可在10.1007/s11032-023-01401-4获取的补充材料。
