Alhatlani Bader Y
Department of Medical Applied Sciences, Unayzah Community College, Qassim University, Unayzah, Saudi Arabia.
Future Virol. 2020 Jul;15(7):409-417. doi: 10.2217/fvl-2020-0163.
The aim of this study was to computationally predict conserved RNA sequences and structures known as -acting RNA elements (CREs) in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome.
MATERIALS & METHODS: Bioinformatics tools were used to analyze and predict CREs by obtaining viral sequences from available databases.
Computational analysis revealed the presence of RNA stem-loop structures within the 3' end of the ORF1ab region analogous to previously identified SARS-CoV genomic packaging signals. Alignment-based RNA secondary structure predictions of the 5' end of the SARS-CoV-2 genome also identified conserved CREs.
These CREs may be potential vaccine and/or antiviral therapeutic targets; however, further studies are warranted to confirm their roles in the SARS-CoV-2 life cycle.
本研究的目的是通过计算预测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）基因组中被称为顺式作用RNA元件（CRE）的保守RNA序列和结构。
利用生物信息学工具，通过从可用数据库中获取病毒序列来分析和预测CRE。
计算分析显示，在ORF1ab区域的3'端存在类似于先前鉴定的SARS-CoV基因组包装信号的RNA茎环结构。基于比对的SARS-CoV-2基因组5'端RNA二级结构预测也鉴定出了保守的CRE。
这些CRE可能是潜在的疫苗和/或抗病毒治疗靶点；然而，需要进一步研究以证实它们在SARS-CoV-２生命周期中的作用。
