Sun Shengyi, Zhao Yiding, Shuai Ling
State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300350, China.
Tate Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Comput Struct Biotechnol J. 2020 Sep 12;18:2471-2479. doi: 10.1016/j.csbj.2020.09.006. eCollection 2020.
Mammalian haploid cells provide insights into multiple genetics approaches as have been proved by advances in homozygous phenotypes and function as gametes. Recent achievements make ploidy of mammalian haploid cells stable and improve the developmental efficiency of embryos derived from mammalian haploid cells intracytoplasmic microinjection, which promise great potentials for using mammalian haploid cells in forward and reverse genetic screening. In this review, we introduce breakthroughs of mammalian haploid cells involving in mechanisms of self-diploidization, forward genetics for various targeting genes and imprinted genes related development.
哺乳动物单倍体细胞为多种遗传学方法提供了深入见解，这已通过纯合表型的进展以及作为配子的功能得到证明。最近的成果使哺乳动物单倍体细胞的倍性稳定，并提高了源自哺乳动物单倍体细胞胞浆内显微注射的胚胎的发育效率，这为在正向和反向遗传筛选中使用哺乳动物单倍体细胞带来了巨大潜力。在本综述中，我们介绍了哺乳动物单倍体细胞在自我二倍体化机制、各种靶向基因的正向遗传学以及与印记基因相关的发育方面的突破。
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