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Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon.

作者信息

Mao Junjie, Xu Kai, Han Jiabao, Feng Guihai, Zhang Ying, Li Wei

机构信息

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, 100101, China.

出版信息

Protein Cell. 2020 Jun;11(6):452-457. doi: 10.1007/s13238-020-00702-0.

DOI:10.1007/s13238-020-00702-0
PMID:32193872
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7251005/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13cc/7251005/2c6836a0a7fe/13238_2020_702_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13cc/7251005/5280488cc831/13238_2020_702_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13cc/7251005/2c6836a0a7fe/13238_2020_702_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13cc/7251005/5280488cc831/13238_2020_702_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13cc/7251005/2c6836a0a7fe/13238_2020_702_Fig2_HTML.jpg

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Comput Struct Biotechnol J. 2020 Sep 12;18:2471-2479. doi: 10.1016/j.csbj.2020.09.006. eCollection 2020.

本文引用的文献

1
Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening.利用小分子生成小鼠单倍体体细胞用于全基因组遗传筛选
Cell Rep. 2017 Aug 29;20(9):2227-2237. doi: 10.1016/j.celrep.2017.07.081.
2
The physiological role of drug transporters.药物转运体的生理作用。
Protein Cell. 2015 May;6(5):334-50. doi: 10.1007/s13238-015-0148-2. Epub 2015 Mar 24.
3
Using next-generation sequencing to isolate mutant genes from forward genetic screens.利用下一代测序技术从正向遗传学筛选中分离突变基因。
Nat Rev Genet. 2014 Oct;15(10):662-76. doi: 10.1038/nrg3745. Epub 2014 Aug 20.
4
Genetic exploration of the exit from self-renewal using haploid embryonic stem cells.利用单倍体胚胎干细胞对自我更新退出机制进行遗传学探索。
Cell Stem Cell. 2014 Mar 6;14(3):385-93. doi: 10.1016/j.stem.2013.12.008. Epub 2014 Jan 9.
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Genetic screens in human cells using the CRISPR-Cas9 system.利用 CRISPR-Cas9 系统在人类细胞中进行遗传筛选。
Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
6
Androgenetic haploid embryonic stem cells produce live transgenic mice.雄原单倍体胚胎干细胞可产生活体转基因小鼠。
Nature. 2012 Oct 18;490(7420):407-11. doi: 10.1038/nature11435. Epub 2012 Sep 30.
7
Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation.抑制糖原合酶激酶-3可减轻 Tcf3 对多能性网络的抑制作用,并提高胚胎干细胞对分化的抵抗力。
Nat Cell Biol. 2011 Jun 19;13(7):838-45. doi: 10.1038/ncb2267.
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Integrative genomics viewer.整合基因组浏览器。
Nat Biotechnol. 2011 Jan;29(1):24-6. doi: 10.1038/nbt.1754.
9
Slingshot: a PiggyBac based transposon system for tamoxifen-inducible 'self-inactivating' insertional mutagenesis.弹弓:一种基于 PiggyBac 的转座子系统,用于他莫昔芬诱导的“自我失活”插入性突变。
Nucleic Acids Res. 2010 Oct;38(18):e173. doi: 10.1093/nar/gkq658. Epub 2010 Aug 5.
10
A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells.基于piggyBac转座子的全基因组插入突变的Blm缺陷型小鼠胚胎干细胞文库。
Genome Res. 2009 Apr;19(4):667-73. doi: 10.1101/gr.085621.108. Epub 2009 Feb 20.