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Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon.

作者信息

Mao Junjie, Xu Kai, Han Jiabao, Feng Guihai, Zhang Ying, Li Wei

机构信息

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, 100101, China.

出版信息

Protein Cell. 2020 Jun;11(6):452-457. doi: 10.1007/s13238-020-00702-0.

Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13cc/7251005/5280488cc831/13238_2020_702_Fig1_HTML.jpg

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本文引用的文献

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Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening.
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A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells.
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