转录组分析揭示了甜樱桃（Prunus avium L.）果实对李属坏死环斑病毒（HSVd）反应的相关见解。

Transcriptomic analysis reveals insights into the response to (HSVd) in sweet cherry ( L.) fruits.

作者信息

Xu Li, Zong Xiaojuan, Wang Jiawei, Wei Hairong, Chen Xin, Liu Qingzhong

机构信息

Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, Shandong, People's Republic of China.

出版信息

PeerJ. 2020 Sep 21;8:e10005. doi: 10.7717/peerj.10005. eCollection 2020.

DOI:10.7717/peerj.10005

PMID:33005494

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7513744/

Abstract

(HSVd) is a member of the genus of the family Pospiviroidae and has been found in a wide range of herbaceous and woody hosts. It causes serious dapple fruit symptoms on infected sweet cherry, notably inducing cherry tree decay. In order to better understand the molecular mechanisms of HSVd infection in sweet cherry fruit, transcriptome analysis of HSVd-infected and healthy sweet cherry fruits was carried out. A total of 1,572 differentially expressed genes (DEGs) were identified, involving 961 upregulated DEGs and 611 downregulated DEGs. Functional analysis indicated that the DEGs were mainly involved in plant hormone signal transduction, plant-pathogen interactions, secondary metabolism, and the MAPK signaling pathway. In addition, C2H2 zinc finger, MYB, bHLH, AP2/ERF, C2C2-dof, NAC and WRKY transcription factors can respond to HSVd infection. In order to confirm the high-throughput sequencing results, 16 DEGs were verified by RT-qPCR analysis. The results provided insight into the pathways and genes of sweet cherry fruit in response to HSVd infection.

摘要

李属坏死环斑病毒（HSVd）是马铃薯纺锤块茎类病毒科李属病毒属的成员，已在多种草本和木本寄主中被发现。它会在感染的甜樱桃上引发严重的果实斑驳症状，尤其会导致樱桃树腐烂。为了更好地了解HSVd在甜樱桃果实中感染的分子机制，对感染HSVd和健康的甜樱桃果实进行了转录组分析。共鉴定出1572个差异表达基因（DEGs），其中包括961个上调的DEGs和611个下调的DEGs。功能分析表明，这些DEGs主要参与植物激素信号转导、植物-病原体相互作用、次生代谢以及丝裂原活化蛋白激酶（MAPK）信号通路。此外，C2H2锌指、MYB、bHLH、AP2/ERF、C2C2-锌指蛋白、NAC和WRKY转录因子能够对HSVd感染作出反应。为了证实高通量测序结果，通过逆转录定量聚合酶链反应（RT-qPCR）分析对16个DEGs进行了验证。这些结果为甜樱桃果实响应HSVd感染的途径和基因提供了深入了解。