Ueda R, Obata Y, Takahashi T
Gan To Kagaku Ryoho. 1987 Jun;14(6 Pt 2):2176-84.
The analysis of cell surface markers with monoclonal antibodies has recently been developed and has been proved to be valuable in the diagnosis and classification of hematopoietic tumors. Occasionally, however, such serological studies have been shown to be inconclusive in identifying cell lineage and or clonal proliferation. In order to overcome these problems, two new approaches were introduced in our laboratory. First, T cell receptor and immunoglobulin gene rearrangement analysis as a means of immunomolecular marking was carried out. Second, a double immunoenzymatic staining technique for determining the surface phenotypes of proliferating lymphocytes using a monoclonal antibody against DNA polymerase alpha together with those detecting lymphocyte membrane antigens was developed. The results revealed by these techniques strongly suggested that some CD2-, CD5+, CD7+ ALL cases are of T cell origin and that AILD may be a neoplastic disease derived from either of the subsets of peripheral T cells.
利用单克隆抗体分析细胞表面标志物的方法近年来得到了发展，并已被证明在造血肿瘤的诊断和分类中具有重要价值。然而，偶尔也会出现这样的情况，即此类血清学研究在确定细胞谱系和/或克隆增殖方面并无定论。为了克服这些问题，我们实验室引入了两种新方法。首先，进行了T细胞受体和免疫球蛋白基因重排分析，作为免疫分子标记的一种手段。其次，开发了一种双重免疫酶染色技术，使用抗DNA聚合酶α的单克隆抗体以及检测淋巴细胞膜抗原的抗体来确定增殖淋巴细胞的表面表型。这些技术所揭示的结果有力地表明，一些CD2-、CD5+、CD7+急性淋巴细胞白血病病例起源于T细胞，而且成人T细胞白血病可能是一种源自外周T细胞亚群之一的肿瘤性疾病。
