澳大利亚首次报告的A的分子特征。
Molecular characterization of the first reported A in Australia.
作者信息
Northill Judith A, Simmons Russell J, Genge Doris, Moore Frederick A
机构信息
Public Health Virology, Forensic and Scientific Services, Coopers Plains, QLD, Australia.
出版信息
Access Microbiol. 2020 Feb 11;2(4):acmi000099. doi: 10.1099/acmi.0.000099. eCollection 2020.
A novel real-time reverse transcription polymerase chain reaction (RT-rPCR) assay was developed to detect (AiV-A) based on four complete genomes. The assay successfully detected AiV-A in a sample from a patient with acute gastroenteritis in January 2008. Screening of 756 samples submitted for norovirus testing during May 2008 detected a further 23 AiV-A-positive samples from 18 individual patients. Genotyping using novel primers targeting the 3C-3D junction region identified AiV-A genotype B. Further sequencing of the VP1 region supported the 3C-3D result. All three assays proved useful to support foodborne outbreak investigations. This is the first report of AiV-A detection in Australia.
基于四个完整基因组开发了一种新型实时逆转录聚合酶链反应（RT-rPCR）检测方法，用于检测戊型肝炎病毒A（AiV-A）。该检测方法于2008年1月成功检测出一名急性肠胃炎患者样本中的AiV-A。在2008年5月提交进行诺如病毒检测的756份样本筛查中，又从18名个体患者中检测出23份AiV-A阳性样本。使用针对3C-3D连接区域的新型引物进行基因分型，确定为AiV-A基因型B。对VP1区域的进一步测序支持了3C-3D的结果。所有这三种检测方法都被证明有助于支持食源性疾病暴发调查。这是澳大利亚关于AiV-A检测的首次报告。
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