DeLellis R A, Wolfe H J
Arch Pathol Lab Med. 1987 Jul;111(7):620-7.
Many of the new technologies that have developed as a result of advances in cellular and molecular biology, genetics, and immunology have had a major impact in clinical medicine and will continue to provide important diagnostic and investigative tools in the future. The ability to localize a wide variety of gene products in tissues has been accomplished largely through the use of immunohistochemistry. Characterization of gene expression at the levels of specific messenger RNAs and genomic DNAs is now possible through the use of both blot and in situ hybridization techniques. These molecular biological technologies provide important new approaches for understanding the pathobiology of disease and for providing more objective and rational diagnostic and prognostic criteria in biopsy specimens. The pathologist is in a unique position to coordinate, implement, and lead efforts utilizing these new methods for the study of human disease. In addition, the pathologist must assume a leadership role in the assessment of the efficacies of the new technologies in resolving specific clinical problems and in providing novel approaches for clinical and basic research.
许多因细胞与分子生物学、遗传学及免疫学进展而发展起来的新技术，已对临床医学产生了重大影响，并将在未来继续提供重要的诊断和研究工具。在组织中定位多种基因产物的能力在很大程度上是通过免疫组织化学实现的。现在，通过印迹法和原位杂交技术，能够在特定信使核糖核酸和基因组脱氧核糖核酸水平上对基因表达进行表征。这些分子生物学技术为理解疾病的病理生物学以及为活检标本提供更客观、合理的诊断和预后标准提供了重要的新方法。病理学家处于独特的地位，能够协调、实施并引领利用这些新方法研究人类疾病的工作。此外，病理学家必须在评估新技术解决特定临床问题的效果以及为临床和基础研究提供新方法方面发挥领导作用。
