Elgarhy Lamia Hamouda, Nofal Ola Elgharably, El-Shorbagy Safinaz Hamdi, Abdel-Latif Amany Mohamed
Department of Dermatology and Venereology, Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Department of Pathology, Faculty of Medicine, Tanta University, Tanta, Egypt.
Dermatol Ther. 2020 Nov;33(6):e14362. doi: 10.1111/dth.14362. Epub 2020 Oct 19.
There is a continuous need for modifications of epidermal cell grafting techniques treating stable vitiligo to make it easier, more economic, and with better outcome. To evaluate the efficacy and safety of noncultured, nontrypsinized epidermal cell graft homogenized with plasma gel, followed by narrow band-ultraviolet B (NB-UVB) in treatment of stable vitiligo, about 40 patients with stable vitiligo underwent harvesting of epidermal cells from the donor site by dermabrasion then the harvested cells were prepared, homogenized with autologous plasma gel, and applied to the abraded recipient, followed by 16 NB-UVB sessions after complete healing. Patches within the same anatomical site received only NB-UVB as controls. The percentage of improvement ranged from 23.33% to 100% with more than 75% uniform, homogenous repigmentation in 65% of patients. Donor site healed completely with normal skin. Noncultured, nontrypsinized epidermal cell grafting technique homogenized with plasma gel followed by NB-UVB sessions gave positive responses and was well tolerated in different body sites in stable resistant vitiligo.
持续需要改进治疗稳定型白癜风的表皮细胞移植技术，使其更简便、更经济且疗效更佳。为评估经血浆凝胶匀化的非培养、非胰蛋白酶处理的表皮细胞移植联合窄谱中波紫外线（NB-UVB）治疗稳定型白癜风的疗效和安全性，约40例稳定型白癜风患者通过磨皮从供皮区采集表皮细胞，然后制备采集的细胞，与自体血浆凝胶匀化，并应用于磨皮后的受皮区，完全愈合后进行16次NB-UVB照射。同一解剖部位的皮损仅接受NB-UVB照射作为对照。改善率在23.33%至100%之间，65%的患者有超过75%的均匀、同质色素再生。供皮区完全愈合，皮肤正常。经血浆凝胶匀化后进行NB-UVB照射的非培养、非胰蛋白酶处理的表皮细胞移植技术在稳定型难治性白癜风的不同身体部位均产生了阳性反应，且耐受性良好。
