Faculty of Technology and Metallurgy, University of Belgrade, Innovation Center, Belgrade, Serbia.
Department of Biology, University of Selcuk, Faculty of Science, Konya, Turkey.
J Sci Food Agric. 2021 Apr;101(6):2284-2293. doi: 10.1002/jsfa.10849. Epub 2020 Oct 22.
This study provides an insight into the impact of ultrasound-assisted extraction with water as solvent (UAEW) and extraction by supercritical carbon dioxide (SC-CO ) with 5% EtOH on antioxidant and enzyme inhibitory activity in regard to the chemical profile of the edible and medicinal mushroom, Pleurotus pulmonarius.
Extraction efficiency was between 0.36% and 63.32%, depending on the extraction technique. The main compounds in the extracts were fatty acids. Supercritical CO extraction with co-solvent was the most suitable method for obtaining extracts that were rich in ergosterol content, reaching a value of 40.1 mg g . The UAEW of crude mushroom powder ensured the highest yield, as well as the extracts with best antioxidative activity. The measurements of enzyme inhibitory activity revealed that all types of investigated extracts exhibited only tyrosinase and amylase inhibition at a significant level.
Based on our results, the extraction methods significantly affected the chemical profile and bioactivity of P. pulmonarius. © 2020 Society of Chemical Industry.
本研究深入探讨了以水为溶剂的超声辅助提取（UAEW）和以 5%乙醇的超临界二氧化碳（SC-CO ）提取对食用药用蘑菇肺形侧耳（Pleurotus pulmonarius）的化学特征的抗氧化和酶抑制活性的影响。
提取效率取决于提取技术，在 0.36%至 63.32%之间。提取物中的主要化合物为脂肪酸。用共溶剂进行的超临界 CO 萃取是获得富含麦角固醇含量提取物的最适宜方法，达到 40.1mg/g 的含量。粗蘑菇粉的 UAEW 确保了最高的收率，以及具有最佳抗氧化活性的提取物。酶抑制活性的测量表明，所有类型的提取物均仅对酪氨酸酶和淀粉酶表现出显著的抑制作用。
根据我们的结果，提取方法显著影响了肺形侧耳的化学特征和生物活性。© 2020 化学工业协会。
