LABEX/FEA (Extraction Laboratory/Faculty of Food Engineering), ITEC (Institute of Technology), UFPA (Federal University of Para), Rua Augusto Corrêa S/N, Guamá, 66075-900 Belém, PA, Brazil.
LAPOA/FEA (Laboratory of Products of Animal Origin/Faculty of Food Engineering), ITEC (Institute of Technology), UFPA (Federal University of Para), Rua Augusto Corrêa S/N, Guamá, 66075-900 Belém, PA, Brazil.
Food Chem. 2020 Nov 15;330:127173. doi: 10.1016/j.foodchem.2020.127173. Epub 2020 May 29.
Pleurotus ostreatus is an edible mushroom with pharmacological potential, due to its metabolites, mainly polysaccharides. On here, the development of a new methodology for the recovery of extract rich in antioxidant polysaccharide was reported. The extracts were characterized, evaluated for antioxidant activity in vitro and in cell models and cytotoxicity. The best defined extraction condition was 25 MPa, 433.15 K, and 20% HO, with 30.69% of the total yield and 0.921 mg of CHO. The anomeric bonds, identified in the FTIR and NMR spectrum, indicate that the extracts are a mixture of heteropolysaccharides, β-glucans, α-glucans, and oligosaccharides. The best extraction condition has 80.83% of antioxidant activity, without cytotoxic effect in vitro. In addition to antioxidant activity in cell model, increasing protection against oxidative damage induced by HO. Finally, HO + CO-SFE technology can be used to obtain extracts rich in antioxidant polysaccharides with pharmacological and food potential.
糙皮侧耳是一种具有药用潜力的食用蘑菇，其代谢产物主要为多糖。在此，报道了一种新的富含抗氧化多糖提取物的提取方法。对提取物进行了表征，并在体外和细胞模型中评估了抗氧化活性和细胞毒性。最佳提取条件为 25 MPa、433.15 K 和 20% H2O2，总得率为 30.69%，CHO 为 0.921mg。在 FTIR 和 NMR 谱中鉴定出的糖苷键表明，提取物是一种混合的杂多糖、β-葡聚糖、α-葡聚糖和低聚糖。最佳提取条件下具有 80.83%的抗氧化活性，体外无细胞毒性。此外，在细胞模型中还具有抗氧化活性，能增加对 HO 诱导的氧化损伤的保护作用。最后，HO+CO-SFE 技术可用于获得具有药理和食品潜力的富含抗氧化多糖的提取物。
