Jauregui H O
ASAIO Trans. 1987 Apr-Jun;33(2):66-74.
This presentation has covered, in part, the subject of fibroblasts or parenchymal cell adhesion to biomaterials and the manipulation of biomaterial surfaces by appropriate biologic coatings from the viewpoint of the biologist. A tissue culture laboratory with good biochemical instrumentation can contribute greatly to the understanding of biomaterial-cell interactions. Such a laboratory cannot only test the toxicity of polymers, but can also proceed with systematic investigations of the role of synthetic and biologic substrates in favoring non-transformed cell attachment, improving cell spreading, and prolonging the life in vitro of normal cell constituents from a diversity of human tissues or organs. Yet, there are certain questions about the biomaterial-tissue interactions that will only be answered by in vivo experiments, since in vitro data may or may not predict the in vivo attachment of a particular cell in a highly protein-competitive environment. One important, albeit neglected, fact is that the early deposition of a protein/glycoprotein/lipid layer from serum, plasma, or peritoneal fluid will modify any synthetic surface introduced into the body. Baier called this early step "interface conversion," and it is quite clear that the shape, surface contamination, and physiochemical properties of the synthetic substratum triggers many events that take place during this "interface conversion." Many investigators have tried to explain cell behavior in vitro by manipulating polymer surface charges, hydrophilicity/hydrophobicity, physical and chemical anisotropy, and/or substrate contractility. We believe that new biomaterial surface coatings will be available as soon as new methods of extraction from biomatrices or extracellular matrices become more familiar. These new coatings will probably promote attachment in more specific terms.(ABSTRACT TRUNCATED AT 250 WORDS)
从生物学家的角度来看，本报告部分涵盖了成纤维细胞或实质细胞与生物材料的粘附以及通过适当的生物涂层对生物材料表面进行操控的主题。配备良好生化仪器的组织培养实验室对理解生物材料与细胞的相互作用有很大帮助。这样的实验室不仅可以测试聚合物的毒性，还能够系统研究合成和生物基质在促进未转化细胞附着、改善细胞铺展以及延长来自各种人体组织或器官的正常细胞成分的体外存活时间方面的作用。然而，关于生物材料与组织的相互作用存在一些问题，只有通过体内实验才能解答，因为体外数据可能无法预测特定细胞在高蛋白竞争环境中的体内附着情况。一个重要但被忽视的事实是，血清、血浆或腹膜液中蛋白质/糖蛋白/脂质层的早期沉积会改变引入体内的任何合成表面。拜尔将这一早期步骤称为“界面转变”，很明显，合成基质的形状、表面污染和物理化学性质引发了在这个“界面转变”过程中发生的许多事件。许多研究人员试图通过操控聚合物表面电荷、亲水性/疏水性、物理和化学各向异性以及/或基质收缩性来解释体外细胞行为。我们相信，一旦从生物基质或细胞外基质中提取的新方法更加为人所熟知，新的生物材料表面涂层将会出现。这些新涂层可能会更具体地促进细胞附着。（摘要截选至250词）
