Yoshikawa Orion, Basoli Valentina, Boschetto Francesco, Rondinella Alfredo, Lanzutti Alex, Zhu Wenliang, Greco Enrico, Thieringer Florian Markus, Xu Huaizhong, Marin Elia
Ceramic Physics Laboratory, Faculty of Materials Science and Engineering, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan.
Medical Additive Manufacturing Research Group (Swiss MAM), Department of Biomedical Engineering, University of Basel, Hegenheimermattweg 167C, 4123 Allschwil, Switzerland.
Polymers (Basel). 2024 May 11;16(10):1371. doi: 10.3390/polym16101371.
In this study, electrospun scaffolds were fabricated using polycaprolactone (PCL) loaded with varying concentrations of β-carotene (1.2%, 2.4%, and 3.6%) via the electrospinning technique. The electrospinning process involved the melting of PCL in acetic acid, followed by the incorporation of β-carotene powder under constant stirring. Raman spectroscopy revealed a homogeneous distribution of β-carotene within the PCL matrix. However, the β-carotene appeared in particulate form, rather than being dissolved and blended with the PCL matrix, a result also confirmed by thermogravimetric analysis. Additionally, X-ray diffraction analysis indicated a decrease in crystallinity with increasing β-carotene concentration. Mechanical testing of the scaffolds demonstrated an increase in ultimate strain, accompanied by a reduction in ultimate stress, indicating a potential plasticizing effect. Moreover, antimicrobial assays revealed a marginal antibacterial effect against for scaffolds with higher β-carotene concentrations. Conversely, preliminary biological assessment using KUSA-A1 mesenchymal cells indicated enhanced cellular proliferation in response to the scaffolds, suggesting the potential biocompatibility and cell-stimulating properties of β-carotene-loaded PCL scaffolds. Overall, this study provides insights into the fabrication and characterization of electrospun PCL scaffolds containing β-carotene, laying the groundwork for further exploration in tissue engineering and regenerative medicine applications.
在本研究中，通过静电纺丝技术制备了负载不同浓度β-胡萝卜素（1.2%、2.4%和3.6%）的聚己内酯（PCL）静电纺丝支架。静电纺丝过程包括将PCL在乙酸中熔融，然后在持续搅拌下加入β-胡萝卜素粉末。拉曼光谱显示β-胡萝卜素在PCL基质中分布均匀。然而，β-胡萝卜素呈颗粒状出现，而非溶解并与PCL基质混合，热重分析也证实了这一结果。此外，X射线衍射分析表明，随着β-胡萝卜素浓度的增加，结晶度降低。支架的力学测试表明极限应变增加，同时极限应力降低，表明存在潜在的增塑作用。此外，抗菌试验显示，β-胡萝卜素浓度较高的支架对[此处原文缺失具体受试对象]具有轻微的抗菌作用。相反，使用KUSA-A1间充质细胞进行的初步生物学评估表明，支架能够促进细胞增殖，这表明负载β-胡萝卜素的PCL支架具有潜在的生物相容性和细胞刺激特性。总体而言，本研究为含β-胡萝卜素的PCL静电纺丝支架的制备和表征提供了见解，为组织工程和再生医学应用的进一步探索奠定了基础。
