Huang Xuemin, Lan Maojin, Wang Jian, Guo Longhua, Lin Zhenyu, Sun Ning, Wu Cuimin, Qiu Bin
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel Farming and Processing, Fuzhou University, Fuzhou, Fujian, 350108, PR China.
The State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Biosens Bioelectron. 2020 Dec 1;169:112655. doi: 10.1016/j.bios.2020.112655. Epub 2020 Sep 24.
Benefit from the efficient energy transfer, aggregation-induced emission (AIE) and host-guest recognition as strategies of signal amplification and specific binding have been applied to establish the sensing system; however, the application of these two attractive strategies in one system is rare. Herein, we propose a "turn-on" to "turn-off" fluorescent strategy for sensitive detection of β-galactosidase (β-Gal) based on the application of AIE and host-guest recognition. In this work, a novel red-emitted (635 nm) copper nanoclusters (CuNCs) protected by dithioerythritol (DTE) and β-cyclodextrin (β-CD) has been found to possess AIE property induced by aluminum cations to achieve the "turn-on" process, and the coordinated behavior between aluminum cations and DTE/β-CD CuNCs is also discussed. As the hydrolysis product of β-Gal and 4-nitrophenyl-β-D-galactopyranoside, p-nitrophenol can combine with β-CD in DTE/β-CD CuNCs by the host-guest recognition to realize the efficient photoelectron transfer to "turn-off" the fluorescence. This strategy performs the exciting linear range of 0.0-50.0 U/L and detection limit of 0.56 U/L (S/N = 3) for sensitive detection of β-Gal and further applies in biologic samples successfully. As far as we know, this is the first work to combine AIE and host-guest recognition in one system to construct the sensing platform, which can achieve efficient and specific energy transfer to improve the sensitivity of the system.
受益于高效的能量转移，聚集诱导发光（AIE）以及主客体识别作为信号放大和特异性结合策略已被应用于建立传感系统；然而，将这两种有吸引力的策略应用于一个系统的情况却很少见。在此，我们基于AIE和主客体识别的应用，提出了一种用于灵敏检测β-半乳糖苷酶（β-Gal）的从“开启”到“关闭”的荧光策略。在这项工作中，发现一种由二硫苏糖醇（DTE）和β-环糊精（β-CD）保护的新型红色发射（635nm）铜纳米簇（CuNCs）具有由铝阳离子诱导的AIE特性以实现“开启”过程，并且还讨论了铝阳离子与DTE/β-CD CuNCs之间的配位行为。作为β-Gal和4-硝基苯基-β-D-吡喃半乳糖苷的水解产物，对硝基苯酚可通过主客体识别与DTE/β-CD CuNCs中的β-CD结合，以实现有效的光电子转移从而“关闭”荧光。该策略在灵敏检测β-Gal时表现出0.0 - 50.0 U/L的令人兴奋的线性范围和0.56 U/L（S/N = 3）的检测限，并成功应用于生物样品中。据我们所知，这是首次在一个系统中结合AIE和主客体识别来构建传感平台，该平台可实现高效且特异性的能量转移以提高系统的灵敏度。
