School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, PR China.
Anal Chim Acta. 2022 Mar 15;1198:339554. doi: 10.1016/j.aca.2022.339554. Epub 2022 Jan 26.
An AIE fluorescent probe (TPh-PyBz-β-gal) was designed and synthesized for β-gal detection based on a novel AIE fluorophore and receptor unit D-galactose residue connected by glycosidic bond. The probe exhibited selective and sensitive turn-on fluorescent responses toward β-gal with detection limit reaching to 0.22 U/mL in aqueous environment. After studied from measured data, we proposed that a possible recognition mechanism probably resulted from the cleavage of glycosidic bond along with obviously increasing fluorescent signal at 606 nm. Meanwhile, TPh-PyBz-β-gal showed low cytotoxicity, and could be successfully used for β-gal imaging in cells.
一种基于新型 AIE 荧光团和受体单元 D-半乳糖残基通过糖苷键连接的 AIE 荧光探针（TPh-PyBz-β-gal）被设计并合成用于β-半乳糖检测。该探针对β-半乳糖具有选择性和灵敏的开启型荧光响应，在水相环境中的检测限达到 0.22 U/mL。通过对测量数据的研究，我们提出了一种可能的识别机制，可能是由于糖苷键的断裂以及在 606nm 处荧光信号的明显增强。同时，TPh-PyBz-β-gal 表现出低细胞毒性，并可成功用于细胞中的β-半乳糖成像。
