Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, CC 22, CP 2300 Rafaela, Santa Fe, Argentina.
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, CC 22, CP 2300 Rafaela, Santa Fe, Argentina.
Ticks Tick Borne Dis. 2021 Jan;12(1):101573. doi: 10.1016/j.ttbdis.2020.101573. Epub 2020 Sep 24.
Specimens of a Trypanosoma sp. were found in a haemolymph sample of Rhipicephalus microplus from Argentina. Polymerase chain reaction (PCR) was done targeting the SSU rRNA gene of Trypanosoma spp. and a fragment of 2300 base pairs (bp) was amplified, subsequently a phylogenetic analysis was conducted, based on an alignment of 905 bp, containing the sequence of the Argentina isolate and sequences of different Trypanosoma species retrieved from GenBank. Phylogenetic analysis revealed that this trypanosome is not related to Trypanosoma theileri as was previously thought, instead the strain of Trypanosoma detected in this study can be provisionally determined as belonging to the recently described organism Trypanosoma rhipicephalis. Furthermore, phylogenetic analysis performed in this work revealed that T. rhipicephalis belongs to a novel clade of tick-related trypanosomes, most with limited genetic data, for which essential aspects of both the vertebrate and invertebrate life cycles are lacking. The lack of basic information restricts the inferences that can be done from the present finding and, in addition, points out a clear knowledge gap in the biology of this group of trypanosomes.
从阿根廷的璃眼蜱血淋巴样本中发现了一种锥虫的标本。针对锥虫 SSU rRNA 基因进行了聚合酶链反应（PCR），扩增出了 2300 个碱基对（bp）的片段，随后进行了系统发育分析，基于对包含阿根廷分离株序列和从 GenBank 检索到的不同锥虫物种序列的 905bp 序列的比对。系统发育分析表明，这种锥虫与先前认为的泰勒锥虫无关，而是本研究中检测到的锥虫菌株可暂时确定属于最近描述的虫体，即璃眼蜱锥虫。此外，本研究进行的系统发育分析表明，璃眼蜱锥虫属于一个新的蜱相关锥虫进化枝，其中大多数遗传数据有限，其脊椎动物和无脊椎动物生活史的重要方面都缺乏。基本信息的缺乏限制了从目前的发现中可以得出的推论，此外，还指出了这组锥虫生物学中的一个明显知识空白。
