Department of Medical Microbiology and Parasitology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia.
Higher Institution of Centre of Excellence (HICoE), Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, Kuala Lumpur, Malaysia.
Acta Trop. 2024 Nov;259:107383. doi: 10.1016/j.actatropica.2024.107383. Epub 2024 Sep 6.
A Trypanosoma screening was conducted on 130 pools comprising 1,241 ticks, collected from 674 selected farm ruminants in Peninsular Malaysia. Of these, nine pools were tested positive for Trypanosoma. Subsequent BLAST searches revealed that the 18S rRNA gene sequences were closely related to Trypanosoma rhipicephalis isolate Chaco CB, with percentage similarities ranging from 95.56 % to 99.84 %. Phylogenetic analysis showed that three of the nine sequences formed a clade with Trypanosoma rhipicephalis. The remaining six Trypanosoma sequences formed a distinct clade, separate from T. rhipicephalis and other Trypanosoma species, with genetic distances of 4.34 % and 4.33-4.58 %, respectively. This study marks the first report of tick-associated Trypanosoma in Malaysia and underscores significant research gaps regarding trypanosome interactions with tick hosts in the region.
在马来西亚半岛，从 674 只选定的农场反刍动物中采集了 130 个包含 1241 只蜱的样本进行锥虫筛查。其中，有 9 个样本呈锥虫阳性。随后的 BLAST 搜索显示，18S rRNA 基因序列与查科 CB 的嗜吞噬无形体密切相关，相似度百分比在 95.56%至 99.84%之间。系统发育分析表明，这 9 个序列中有 3 个与嗜吞噬无形体形成一个分支。其余 6 个锥虫序列形成了一个与嗜吞噬无形体和其他锥虫物种不同的分支，遗传距离分别为 4.34%和 4.33-4.58%。本研究首次报道了马来西亚蜱传锥虫，并强调了该地区锥虫与蜱宿主相互作用方面存在重大研究空白。
