Arrazuria Rakel, Knight Cameron G, Lahiri Priyoshi, Cobo Eduardo R, Barkema Herman W, De Buck Jeroen
Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, Canada.
Department of Veterinary Clinical and Diagnostic Sciences, Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, Canada.
Microorganisms. 2020 Sep 30;8(10):1507. doi: 10.3390/microorganisms8101507.
Digital dermatitis (DD) causes lameness in cattle with substantial negative impact on sustainability and animal welfare. Although several species of bacteria have been isolated from various DD stages, their individual or synergistic roles in the initiation or development of lesions remain largely unknown. The objective of this study was to compare effects of the three most common species isolated from DD lesions in cattle (), both as individual and as mixed inoculations, in a murine abscess model. A total of 10 or 5 × 10 spp. were inoculated subcutaneously, and produced abscess was studied after 7 days post infection. There were no synergistic effects when two or three species were inoculated together; however, produced the largest abscesses, whereas those produced by were the smallest and least severe. species were cultured from skin lesions at 7 days post infection and, additionally, from the kidneys of some mice (2/5), confirming systemic infection may occur. Taken together, these findings suggest that and may have more important roles in DD lesion initiation and development than .
指状皮炎（DD）会导致牛跛行，对可持续性和动物福利产生重大负面影响。尽管已从DD的各个阶段分离出几种细菌，但它们在病变起始或发展中的单独或协同作用仍 largely unknown。本研究的目的是在小鼠脓肿模型中比较从牛的DD病变中分离出的三种最常见细菌（）单独接种和混合接种的效果。总共10或5×10个菌种进行皮下接种，感染后7天研究产生的脓肿。两种或三种菌种一起接种时没有协同作用；然而，产生的脓肿最大，而产生的脓肿最小且最不严重。感染后7天从皮肤病变中培养出菌种，此外，还从一些小鼠的肾脏（2/5）中培养出菌种，证实可能发生全身感染。综上所述，这些发现表明，与相比，和在DD病变的起始和发展中可能具有更重要的作用。
