Wachholz Gabriela Elis, Varela Ana Paula Muterle, Teixeira Thais Fumaco, de Matos Sophia Martins Simon, Rigon da Luz Soster Paula, Vianna Fernanda Sales Luiz, de Souza Diogo Onofre Gomes, Roehe Paulo Michel, Schuler-Faccini Lavínia, Fraga Lucas Rosa
Postgraduate Program in Genetics and Molecular Biology, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Teratogen Information Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Birth Defects Res. 2021 Jan 1;113(1):22-31. doi: 10.1002/bdr2.1813. Epub 2020 Oct 3.
Zika virus (ZIKV) was confirmed to be related to microcephaly in 2016. However, there is still a need for understanding the embryonic morphological changes induced by ZIKV and when they occur. Here, chicken embryos were chosen as experimental model of ZIKV to evaluate virus-associated morphological alterations that might take place during embryonic development.
A screening with different viral doses was conducted in embryos at HH Stage 10-12 (E1.5) as well as a follow up of the first 5 days postinfection (dpi) was performed to observe the main morphologic changes post ZIKV infection.
ZIKV exposed embryos presented a higher prevalence of mortality and defects such as brain malformation when compared to controls. Moreover, we observed that the phenotypes become more evident at 4dpi, when the viral load quantification reaches a peak.
We found that ZIKV exposed embryos presented a high prevalence of mortality and central nervous system (CNS) abnormalities in a dose-dependent manner. The phenotype was more evident 4 days postinfection, when the viral load quantification reached a peak.
2016年寨卡病毒（ZIKV）被证实与小头畸形有关。然而，仍需要了解ZIKV诱导的胚胎形态变化以及这些变化何时发生。在此，选择鸡胚作为ZIKV的实验模型，以评估胚胎发育过程中可能发生的与病毒相关的形态改变。
在HH第10-12阶段（E1.5）的胚胎中进行不同病毒剂量的筛选，并在感染后第1天（dpi）的前5天进行随访，以观察ZIKV感染后的主要形态变化。
与对照组相比，暴露于ZIKV的胚胎死亡率更高，且存在脑畸形等缺陷。此外，我们观察到，当病毒载量定量达到峰值时，在4dpi时表型变得更加明显。
我们发现，暴露于ZIKV的胚胎死亡率高，且中枢神经系统（CNS）异常呈剂量依赖性。感染后4天，当病毒载量定量达到峰值时，表型更加明显。
