College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, PR China.
General Monitoring Station for Wildlife-Borne Infectious Diseases, State Forestry and Grass Administration, Shenyang, Liaoning Province, 110034, PR China.
Microb Pathog. 2020 Dec;149:104526. doi: 10.1016/j.micpath.2020.104526. Epub 2020 Sep 30.
Cryptosporidium spp. and Enterocytozoon bieneusi are two important zoonotic pathogens that can cause diarrhea and other gastrointestinal illnesses in humans and animals. However, the prevalence and genotype of the parasites in Longjiang Wagyu cattle in Heilongjiang Province, Northeast China have not been reported. In the present study, a total of 423 fecal samples of Longjiang Wagyu cattle collected from different farms in Heilongjiang Province, Northeast China, were examined for Cryptosporidium spp. and E. bieneusi using nested PCR. The overall infection rates for Cryptosporidium spp. and E. bieneusi were 6.38% (n = 27) and 7.09% (n = 30), respectively. The prevalence in different age groups ranged from 3.80% (95% confidence interval (CI) 1.01-6.59) to 8.36% (95% CI 4.83-11.90) for Cryptosporidium spp. and 5.97% (95% CI 2.52-9.43) to 7.94% (95% CI 4.49-11.40) for E. bieneusi. By analyzing the DNA sequences of the small subunit (SSU) rRNA gene, two Cryptosporidium species were detected in this study, namely C. parvum (n = 25) and C. ryanae (n = 2). The IIdA20G1 subtype was further identified by using the 60-kDa glycoprotein (gp60) gene of C. parvum. E. bieneusi was identified using three known sequences through the analysis of internal transcribed spacer (ITS) sequences: J (n = 23), I (n = 5), and BEB4 (n = 2), and all belonged to group 2. The results indicated that some of the Cryptosporidium species and E. bieneusi genotypes identified in Longjiang Wagyu cattle in the study areas might have zoonotic potential.
隐孢子虫属和肠微孢子虫是两种重要的人畜共患病原体，可导致人类和动物腹泻和其他胃肠道疾病。然而，中国东北黑龙江省龙江和牛的寄生虫流行率和基因型尚未报道。本研究采用巢式 PCR 法检测了来自中国东北黑龙江省不同农场的 423 份龙江和牛粪便样本中的隐孢子虫属和肠微孢子虫。隐孢子虫属和肠微孢子虫的总感染率分别为 6.38%（n=27）和 7.09%（n=30）。不同年龄组的患病率范围为隐孢子虫属 3.80%（95%置信区间（CI）1.01-6.59）至 8.36%（95% CI 4.83-11.90），肠微孢子虫属 5.97%（95% CI 2.52-9.43）至 7.94%（95% CI 4.49-11.40）。通过分析小亚单位（SSU）rRNA 基因的 DNA 序列，本研究检测到两种隐孢子虫，即 C. parvum（n=25）和 C. ryanae（n=2）。通过对 C. parvum 的 60-kDa 糖蛋白（gp60）基因的分析，进一步鉴定出 IIdA20G1 亚型。通过对内部转录间隔区（ITS）序列的分析，用三个已知序列鉴定肠微孢子虫：J（n=23）、I（n=5）和 BEB4（n=2），均属于 2 组。结果表明，本研究地区龙江和牛中一些隐孢子虫种和肠微孢子虫基因型可能具有人畜共患潜力。
