Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Fish Shellfish Immunol. 2020 Dec;107(Pt A):163-170. doi: 10.1016/j.fsi.2020.09.042. Epub 2020 Sep 30.
In this study, the compound antimicrobial peptides was added to the basic diet to probe its effects on grass carp for 8 weeks. There were 6 groups in our study, including M0 (0 mg/kg) and 5 additional groups: M1 (100 mg/kg), M2 (200 mg/kg), M3 (400 mg/kg), M4 (800 mg/kg) and M5 (1600 mg/kg). The results are as follows: (1) The grass carp's FBW, WGR and SGR in M4 were significantly increased than M0 (p < 0.05). (2) In the hepatopancreas, the amylase activity of M2, M3 and M5 were significant up-regulation than other groups (p < 0.05). (3) The activity of T-AOC in the M3 and M4 was significantly higher compared to the other groups in grass carp hepatopancreas, while MDA was significantly reduced compared to the control group (p < 0.05). The activity of SOD in M5 was significantly increased than the other groups (p < 0.05). (4) The expression of MnSOD (except head kidney), IL8 and TNF-α and IL-1β (except hepatopancreas) were significantly increased (p < 0.05), while TGF-β and were significantly reduced in the hepatopancreas, spleen and head kidney at M3 (p < 0.05), and IL10 were significantly decreased in the hepatopancreas at M3 (p < 0.05). In addition, expression of IL8 and TNF-α were significant down-regulation, whereas TGF-β (expect head kidney) were significant up-regulation in hepatopancreas, spleen and head kidney in M3 after challenge with A. hydrophila. The expression of IL-1β in spleen and head kidney were also down-regulated, whereas IL10 were significantly up-regulated in the hepatopancreas at M3 after challenge with A. hydrophila (p < 0.05). The results of this study showed that grass carp fed a diet supplemented compound antimicrobial peptides was added with 400-800 mg/kg, which could improve the growth performance, antioxidant and digestive capabilities, and could also enhance the expression of immune-related genes and control to A. hydrophila.
本研究在基础日粮中添加复合抗菌肽，探究其对草鱼 8 周的影响。共设 6 组，分别为 M0（0mg/kg）和 5 个添加组：M1（100mg/kg）、M2（200mg/kg）、M3（400mg/kg）、M4（800mg/kg）和 M5（1600mg/kg）。结果如下：（1）M4 组的 FBW、WGR 和 SGR 显著高于 M0（p<0.05）。（2）在肝胰腺中，M2、M3 和 M5 组的淀粉酶活性显著上调（p<0.05）。（3）M3 和 M4 组的 T-AOC 活性显著高于其他组，MDA 显著低于对照组（p<0.05）。M5 组的 SOD 活性显著高于其他组（p<0.05）。（4）MnSOD（头肾除外）、IL8 和 TNF-α、IL-1β（肝胰腺除外）的表达显著上调（p<0.05），而 TGF-β在肝胰腺、脾脏和头肾中的表达显著下调（p<0.05），IL10 在肝胰腺中的表达显著下调（p<0.05）。此外，在受到嗜水气单胞菌攻毒后，M3 组的 IL8 和 TNF-α 的表达显著下调，而 TGF-β（头肾除外）的表达显著上调，M3 组的脾脏和头肾的 IL-1β 表达也下调，而 IL10 的表达显著上调（p<0.05）。本研究结果表明，在日粮中添加 400-800mg/kg 的复合抗菌肽可提高草鱼的生长性能、抗氧化和消化能力，并能增强免疫相关基因的表达，控制草鱼对嗜水气单胞菌的感染。
