Ma Fang, Ma Ruilin, Zhao Lei
Key Laboratory of Resource Utilization of Agricultural Solid Waste in Gansu Province, Tianshui Normal University, South Xihe Road, Qinzhou District, Tianshui, 741000, Gansu, People's Republic of China.
Biochem Genet. 2025 Feb;63(1):85-103. doi: 10.1007/s10528-024-10708-6. Epub 2024 Feb 27.
Antimicrobial peptides (AMPs) are an important part of non-specific immunity and play a key role in the cellular host defense against pathogens and tissue injury infections. We investigated the effects of AMP supplementation on the antioxidant capacity, non-specific immunity, and gut microbiota of tsinling lenok trout. 240 fish were fed diets (CT, A120, A240 and A480) containing different amounts of AMP peptides (0, 120 mg kg, 240 mg kg, 480 mg kg) for 8 weeks. Our results showed that the activity of total antioxidant capacity (T-SOD) and glutathione peroxidase (GSH-Px), lysozyme (LZM), catalase (CAT) and acid phosphatase (ACP) in the A240 and A480 group were higher than that in the CT group (P < 0.05). The content of malondialdehyde (MDA) in AMP group was significantly lower than that in CT group (P < 0.05). Furthermore, we harvested the mid-gut and applied next-generation sequencing of 16S rDNA. The results showed that the abundance of Halomonas in AMP group was significantly lower than that in CT group. Functional analysis showed that the abundance of chloroalkane and chloroalkene degradation pathway increased significantly in AMP group. In conclusion, AMP enhanced the antioxidant capacity, non-specific immunity, and intestinal health of tsinling lenok trout.
抗菌肽（AMPs）是非特异性免疫的重要组成部分，在宿主细胞抵御病原体和组织损伤感染中发挥关键作用。我们研究了补充抗菌肽对秦岭细鳞鲑抗氧化能力、非特异性免疫和肠道微生物群的影响。240条鱼被投喂含有不同量抗菌肽（0、120毫克/千克、240毫克/千克、480毫克/千克）的饲料（CT、A120、A240和A480），持续8周。我们的结果表明，A240和A480组的总抗氧化能力（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）、溶菌酶（LZM）、过氧化氢酶（CAT）和酸性磷酸酶（ACP）活性均高于CT组（P<0.05）。抗菌肽组丙二醛（MDA）含量显著低于CT组（P<0.05）。此外，我们采集了中肠并应用16S rDNA进行下一代测序。结果表明，抗菌肽组嗜盐单胞菌的丰度显著低于CT组。功能分析表明，抗菌肽组氯代烷烃和氯代烯烃降解途径的丰度显著增加。总之，抗菌肽增强了秦岭细鳞鲑的抗氧化能力、非特异性免疫和肠道健康。
