Chongqing Research Center for Pharmaceutical Engineering, College of Pharmacy, Chongqing Medical University, Chongqing 400016, PR China.
Research Center for Pharmacodynamic Evaluation Engineering Technology of Chongqing, College of Pharmacy, Chongqing Medical University, Chongqing 400016, PR China.
Food Chem. 2021 Mar 15;340:128128. doi: 10.1016/j.foodchem.2020.128128. Epub 2020 Sep 19.
In this research, a novel signal-on aptasensor for highly sensitive detection of zearalenone (ZEN) was reported based on target-induced amplification strategy. Specifically, chitosan functionalized acetylene black and multi-walled carbon nanotubes (CS@AB-MWCNTs) nanocomposite with large specific surface area and excellent conductivity was synthesized and served as the sensing platform. In addition, carboxylated graphene oxide-labeled ZEN binding aptamer (CGO-ZBA) would specifically recognized with ZEN to detach from the electrode, allowing the electrochemical signal of [Fe(CN)] increased more obviously. Under the optimal conditions, the proposed aptasensor exhibited exceptional detection performances for ZEN with a linear range from 10 fg mL to 1 ng mL and a low limit of detection of 3.64 fg mL. Given its great sensitivity, excellent selectivity, satisfactory stability and reproducibility, this method would provide a promising application for ZEN and other biomolecules by replacing the corresponding nucleicacidsequences.
在这项研究中，我们基于目标诱导扩增策略，报道了一种用于高灵敏检测玉米赤霉烯酮（ZEN）的新型信号开启适体传感器。具体来说，我们合成了具有大比表面积和优异导电性的壳聚糖功能化乙炔黑和多壁碳纳米管（CS@AB-MWCNTs）纳米复合材料，并将其用作传感平台。此外，羧基化石墨烯氧化物标记的 ZEN 结合适体（CGO-ZBA）可以与 ZEN 特异性结合从而从电极上脱离，从而使 [Fe(CN)]的电化学信号更加明显增加。在最佳条件下，所提出的适体传感器对 ZEN 表现出优异的检测性能，线性范围为 10 fg mL 至 1 ng mL，检测限低至 3.64 fg mL。鉴于其高灵敏度、优异的选择性、令人满意的稳定性和重现性，该方法有望通过取代相应的核酸序列，为 ZEN 和其他生物分子的检测提供一种有前途的应用。
